| Journal: | Biocell |
| Database: | PERIÓDICA |
| System number: | 000324478 |
| ISSN: | 0327-9545 |
| Authors: | Fontana, María Laura1 Mroginski, Luis Amado1 Rey, Hebe Yolanda1 |
| Institutions: | 1Universidad Nacional del Nordeste, Instituto de Botánica del Nordeste, Corrientes. Argentina |
| Year: | 2009 |
| Season: | Dic |
| Volumen: | 33 |
| Number: | 3 |
| Pages: | 179-186 |
| Country: | Argentina |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | With the aim of developing an efficient plant regeneration protocol, leaflet explants of three accessions of Arachis villosa Benth. (S2866, S2867 and L97) were cultured on basic Murashige and Skoog medium supplemented with different combinations of plant growth regulators: α-naphthalenacetic acid, indole- 3-butyric acid, 6-benzylaminopurine, kinetin and thidiazuron. The accession L97 was the only one able to differentiate buds through indirect organogenesis. The most suitable combination for bud regeneration was the basic medium added with 13.62 μM thidiazuron and 4.44 μM 6-benzylaminopurine. These results show the important role of the genotype in morphogenetic responses and the organogenetic effect of thidiazuron in Arachis villosa accession L97. A thidiazuron lacking media (only 0.54 μM α-naphthalenacetic acid, 13.95 μM kinetin and 13.32 μM 6-benzylaminopurine were added) promoted the elongation of the regenerated buds. Adventitious rooting was achieved 90 days after the isolated shoots were transferred to a rooting medium containing 0.54 μM α-naphthalenacetic acid |
| Disciplines: | Agrociencias, Biología |
| Keyword: | Leguminosas, Fisiología vegetal, Cultivo de tejidos, Micropropagación vegetal, Explantes foliares, Tidiazurón, Arachis villosa |
| Keyword: | Agricultural sciences, Biology, Legumes, Plant physiology, Tissue culture, Plant micropropagation, Leaf explants, Thidiazuron, Arachis villosa |
| Full text: | Texto completo (Ver PDF) |
