| Journal: | Arquivos de neuro-psiquiatria |
| Database: | PERIÓDICA |
| System number: | 000383083 |
| ISSN: | 0004-282X |
| Authors: | Jun Ding1 Zhengang Ding2 Fang Yuan1 Jianyi Guo1 Hao Chen1 Wenwei Gao1 Ren Wang1 Yi Gu1 Jiong Chen1 Yan Guo1 Hengli Tian1 |
| Institutions: | 1Shangai Jiaotong University, Department of Neurosurgery, Shanghai. China 2Chinese Medicine Hospital of Huangdao District, Department of Neurosurgery, Shandong. China |
| Year: | 2015 |
| Season: | Abr |
| Volumen: | 73 |
| Number: | 4 |
| Pages: | 342-349 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, analítico |
| English abstract | Many studies of protein expression after traumatic brain injury (TBI) have identified biomarkers for diagnosing or determining the prognosis of TBI. In this study, we searched for additional protein markers of TBI using a fluid perfusion impact device to model TBI in S-D rats. Two-dimensional gel electrophoresis and mass spectrometry were used to identify differentially expressed proteins. After proteomic analysis, we detected 405 and 371 protein spots within a pH range of 3-10 from sham-treated and contused brain cortex, respectively. Eighty protein spots were differentially expressed in the two groups and 20 of these proteins were identified. This study validated the established biomarkers of TBI and identified potential biomarkers that could be examined in future work |
| Portuguese abstract | Muitos estudos de expressão proteica após lesão cerebral traumática (LCT) identificam biomarcadores para determinação diagnóstica ou prognóstica do LCT. No presente estudo, foram investigados marcadores proteicos adicionais de LCT, através de um aparelho de impacto no fluxo e perfusão em ratos S-D. Eletroforese bidimensional em gel e espectrometria de massa foram utilizadas para identificar diferentes proteínas expressas. Após a análise proteômica, detectamos marcas de proteínas 405 e 371, com pH variando entre 3-10 no córtex de ratos sham e naqueles com contusão cerebral, respectivamente. Oitenta marcas proteicas foram expressas nos dois grupos e 20 destas proteínas foram identificadas. Este estudo validou o estabelecimento de biomarcadores de LCT e identificou potencial biomarcadores que poderão ser estudados em estudos futuros |
| Disciplines: | Medicina |
| Keyword: | Diagnóstico, Neurología, Lesiones, Cerebro, Traumatismo craneoencefálico, Biomarcadores, Proteómica, Electroforesis en gel, Espectrometría de masas |
| Keyword: | Medicine, Diagnosis, Neurology, Lesions, Brain, Cranioencephalic trauma, Biomarkers, Proteomics, Gel electrophoresis, Mass spectrometry |
| Full text: | Texto completo (Ver HTML) |
