| Journal: | Annals of hepatology |
| Database: | PERIÓDICA |
| System number: | 000415637 |
| ISSN: | 1665-2681 |
| Authors: | Sánchez García, Adriana1 Ríos Ibarra, Clara Patricia1 Rincón Sánchez, Ana Rosa2 Ortiz López, Rocío1 Garza Juárez, Aurora3 Morlett Chávez, Jesús3 Martínez Rodríguez, Herminia1 Rivas Estilla, Ana María1 |
| Institutions: | 1Universidad Autónoma de Nuevo León, Facultad de Medicina, Monterrey, Nuevo León. México 2Universidad de Guadalajara, Centro Universitario de Ciencias de la Salud, Guadalajara, Jalisco. México 3Universidad Autónoma de Coahuila, Escuela de Ciencias Químicas, Saltillo, Coahuila. México |
| Year: | 2013 |
| Season: | Sep-Oct |
| Volumen: | 12 |
| Number: | 5 |
| Pages: | 725-732 |
| Country: | México |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Analítico, descriptivo |
| English abstract | Acetylsalicylic acid (ASA) has been shown to downregulate HCV expression; however, the involved mechanisms are unknown. We used proteomic analysis to compare protein expression profiles between human hepatocarcinoma cells (Huh7) and Huh7-HCV cells harboring expression of non-structural HCV proteins, to elucidate the mechanism(s) involved in ASA-mediated downregulation of HCV replication. Material and methods. Both cell lines were treated or untreated with 4 mM ASA and harvested at 0, 24, 48 and 72 h to isolate total proteins, which were resolved by two-dimensional gel electrophoresis (2DE) to separate them by isoelectric point (pI), followed by fractionation by molecular weight (MW). Gels were scanned and analyzed with PD-Quest software V8.0.1, and proteins were elucidated by the specific pI and MW using TAGIDENT software. Statistics analysis included the t-test. Results and Discussion. Different protein patterns among hepatocytes expressing HCV-proteins in ASA treated and untreated cells were found. Among proteins differentially expressed in Huh7-HCV cells, we found proteins related to cell proliferation (MTMR6, FAM22, HDGF and HCF-1) after 24 h of ASA treatment; and upregulation of angiostatin, PI4KA and STAT-1 after 48 h of treatment. Finally, at 72 h of ASA exposure, we identified overexpression of adenylsuccinate synthase, 2’-3’-di-deoxyadenosine, ubiquitin-protein-ligase E6A, adenylosuccinate-lyase and nibrin (NBN). Conclusion. We found that ASA induces different protein patterns in Huh7-HCV cells promoting activation of proteins involved in cell progression, repair of double strand breaks, proliferation, inhibition of apoptosis and growth stimulation at the same time that it decreased HCV expression |
| Disciplines: | Medicina |
| Keyword: | Gastroenterología, Genética, Bioquímica, Virus de la hepatitis C, Proteómica, Electroforesis bidimensional |
| Keyword: | Medicine, Gastroenterology, Genetics, Biochemistry, Hepatitis C virus, Proteomics, Bidimensional electrophoresis |
| Full text: | Texto completo (Ver PDF) |
