| Journal: | Annals of hepatology |
| Database: | PERIÓDICA |
| System number: | 000415688 |
| ISSN: | 1665-2681 |
| Authors: | Rendón Huerta, Erika P1 Torres Martínez, Ana1 Charles Niño, Claudia2 Rivas Estilla, Ana M3 Páez, A4 Fortoul, Teresa I1 Montaño, Luis F1 |
| Institutions: | 1Universidad Nacional Autónoma de México, Facultad de Medicina, México, Distrito Federal. México 2Universidad Nacional Autónoma de México, Instituto de Investigaciones Biomédicas, México, Distrito Federal. México 3Universidad Autónoma de Nuevo León, Facultad de Medicina, Monterrey, Nuevo León. México 4Instituto Nacional de Cardiología Ignacio Chávez, Departamento de Fisiología, México, Distrito Federal. México |
| Year: | 2013 |
| Season: | Jul-Ago |
| Volumen: | 12 |
| Number: | 4 |
| Pages: | 448-457 |
| Country: | México |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Analítico, descriptivo |
| English abstract | Hepatitis C virus (HCV) infection usually results in long-term viremia. Entry of HCV into the hepatocyte requires claudin-1, -6, -9 and occludin. The efficacy of Pegylated interferon-α (PEG-IFN) treatment against HCV infection increased when ribavirin (RBV) was added to the therapeutic scheme. Our aim was to investigate if PEG-IFN plus RBV regulate claudin expression. Material and methods. HepG2, Huh-7 and Huh-7.5 cells were treated with PEG-IFN-α2a or α2b and/or RBV at different times before obtaining the cytosolic, membrane and cytoskeletal fractions. Claudin-1, 3, 4, 6, and 9, E-cadherin and occludin expression was evaluated by Western blot analysis. Transepithelial electrical resistance (TER) was also determined. Results. Claudin-1, 3, 4, 6, E-cadherin and occludin are constitutively expressed mainly in HepG2 cell membrane. Claudin-1 and E-cadherin cell membrane expression diminished after exposure to PEGIFNα2b (50 ng) + RBV(50 µg); the maximal decrease was observed with 200 ng of PEG-IFNα2b + 200 µg of RBV. The effect was less intense with PEG-IFNα2a. The inhibition of claudin-1 and E-cadherin expression in Huh-7 and Huh-7.5 cells was only observed with 200 ng of PEG-IFNα2b + 200 µg of RBV. TER diminished marginally in the HCV containing hepatoma cells with 200 ng of PEG-IFNα2b + 200 µg of RBV. Claudin-1 mRNA expression level was not affected by the combined treatment. Conclusion. The increased therapeutic efficacy of the PEG-IFNα2b plus RBV treatment could be secondary to the inhibition of claudin-1 and E-cadherin cell membrane expression |
| Disciplines: | Medicina |
| Keyword: | Gastroenterología, Virus de la hepatitis C, Interferón pegilado, Ribavirina, Claudinas, Cadherinas |
| Keyword: | Medicine, Gastroenterology, Hepatitis C virus, Pegylated interferon, Ribavirin, Claudins, Cadherins |
| Full text: | Texto completo (Ver PDF) |
