| Journal: | Annals of hepatology |
| Database: | PERIÓDICA |
| System number: | 000415671 |
| ISSN: | 1665-2681 |
| Authors: | Yu, Heng-Chao1 Bai, Lu2 Yue, Shu-Qiang1 Wang, De-Sheng1 Wang, Lin1 Han, Hua3 Dou, Ke-Feng1 |
| Institutions: | 1Xi-Jing Hospital, Department of Hepatic Surgery,D, Xi'an, Shaanxi. China 2Xi-Jing Hospital, epartment of Clinic Laboratory, Xi'an, Shaanxi. China 3Fourth Military Medical University, Department of Medical Genetics and Developmental Biology, Xi'an, Shaanxi. China |
| Year: | 2013 |
| Season: | Sep-Oct |
| Volumen: | 12 |
| Number: | 5 |
| Pages: | 815-821 |
| Country: | México |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Analítico, descriptivo |
| English abstract | We have previously reported that Notch signaling pathway protects hepatocytes from ischemia/reperfusion (I/R) injury by repressing reactive oxygen species (ROS) production. However, apart from hepatocytes, non-parenchymal cells including vascular endothelia cells, Kupffer cells and hepatic stellate cells are also reported to be involved in hepatic I/R injury. Aim. To clarify the role of Notch signaling in non-parenchymal cells subjected to I/R injury. Materials and methods. Human Umbilical Vein Endothelial Cells (HUVECs), mouse macrophage line RAW264.7 and rat hepatic stellate cell line HSC-T6 were cultured and subjected to I/R injury, respectively. Activation of Notch signaling was assessed by NICD western blot. Then, pharmacological inhibitor (γ-secretase inhibitor GSI) was used to block Notch signaling of related cell lines in vitro. Intracellular ROS was detected and analyzed by FACS and apoptosis was examined by TUNEL staining and Annexin V staining. Results. Notch signaling responded to I/R injury and I/R injury induced activation of Notch signaling in nonparenchymal cells. Notch signal deficiency led to overproduction of ROS and aggravated cell death of non-parenchymal cells subjected to I/R injury. Conclusion. Notch signal protectes non-parenchymal cells from I/R injury by repressing ROS |
| Disciplines: | Medicina |
| Keyword: | Gastroenterología, Lesión hepática, Isquemia-reperfusión, Apoptosis, Especies reactivas de oxígeno |
| Keyword: | Medicine, Gastroenterology, Liver injury, Ischemia-reperfusion, Apoptosis, Oxygen reactive species |
| Full text: | Texto completo (Ver PDF) |
