| Journal: | Annals of hepatology |
| Database: | PERIÓDICA |
| System number: | 000411681 |
| ISSN: | 1665-2681 |
| Authors: | Zepeda Morales, Adelaida Sara M1 Toro Arreola, Susana del1 García Benavides, Leonel1 Bastidas Ramírez, Blanca E1 Fafutis Morris, Mary1 Pereira Suárez, Ana L1 Bueno Topete, Miriam R1 |
| Institutions: | 1Universidad de Guadalajara, Centro Universitario de Ciencias de la Salud, Guadalajara, Jalisco. México |
| Year: | 2016 |
| Season: | May-Jun |
| Volumen: | 15 |
| Number: | 3 |
| Pages: | 418-426 |
| Country: | México |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental |
| English abstract | IL-17, TGF-β1/2 are cytokines involved in the development of kidney, pulmonary and liver fibrosis. However, their expression kinetics in the pathogenesis of cholestatic liver fibrosis have not yet been fully explored. The aim of the study was to analyze the expression of IL-17, RORγt, NKp46, TGF-β1, and TGF-β2 in the liver of rats with bile duct ligation (BDL). Results. Hepatic IL-17A gene expression analyzed by qRT-PCR showed a dramatic increase of 350 and 10 fold, at 8 and 30 days post BDL, respectively. TGFβ1 and TGFβ2 gene expression significantly increased throughout the whole fibrotic process. At the protein level in liver homogenates, IL-17, TGF-β1, and RORγt significantly increased at 8 and 30 days after BDL. Interestingly, a significant increase in the protein levels of TGF-β2 and decrease of NKp46 was observed only 30 days after BDL. Unexpectedly, TGF-β2 exhibited stronger signals than TGF-β1 at the gene expression and protein levels. Histological analysis showed bile duct proliferation and collagen deposition. Conclusions. Our results suggest that pro-fibrogenic cytokines IL-17, TGF-β1 and, strikingly, TGF-β2 might be important players of liver damage in the pathogenesis of early and advanced experimental cholestatic fibrosis. Th17 cells might represent an important source of IL-17, while NK cell depletion may account for the perpetuation of liver damage in the BDL model |
| Disciplines: | Medicina |
| Keyword: | Farmacología, Gastroenterología, Fibrosis, Patogénesis, Citocinas, TGF-beta, Células asesinas, Vías biliares |
| Keyword: | Medicine, Gastroenterology, Pharmacology, Faldaprevir, Hepatitis C, TGF-beta, Natural killer cells, Bile ducts |
| Full text: | Texto completo (Ver PDF) |
