| Journal: | Annals of hepatology |
| Database: | PERIÓDICA |
| System number: | 000418438 |
| ISSN: | 1665-2681 |
| Authors: | Bassiouny, Ahmad R1 Zaky, Amira1 Fawky, Faten1 Kandeel, Kamal M1 |
| Institutions: | 1Alexandria University, Faculty of Science, Alejandría. Egipto |
| Year: | 2011 |
| Season: | Oct-Dic |
| Volumen: | 10 |
| Number: | 4 |
| Pages: | 516-530 |
| Country: | México |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Analítico, descriptivo |
| English abstract | Apurinic/apyrimidinic endonuclease1/ redox factor-1 (APE1/Ref-1) is a multifunctional protein involved in DNA base excision repair and redox regulation of many transcription factors. It is an important pro-survival protein activated in response to oxidative stress. Increased level of this essential redox sensitive protein correlates closely with cellular survival against oxidative insults. Curcumin (diferuloylmethane) a naturally occurring compound derived from turmeric has attracted interest because of its anti-inflammatory, anti-oxidative, and chemopreventive activities. Material and methods. The current study evaluates the in vivo role of curcumin in protecting and treating liver injury and fibrogenesis caused by carbon tetrachloride (CCl4) in rats. It also addresses the possible involvement of the multifunctional protein APE1 in hepatoprotection. Analysis of APE1 expression was performed at mRNA and protein levels by reverse transcriptase (RT)-PCR and western blotting respectively. Profile of HSCs-activation related genes were assayed by RT-PCR and pro-inflammatory cytokines levels were determined by enzyme-linked immune assays. Results. Here we show that oral administration of curcumin was accompanied by a robust increase in APE1 protein and mRNA levels, and improved the histological architecture of rat liver. In addition, curcumin attenuated oxidative stress by increasing the content of hepatic glutathione within normal values, leading to the reduction in the level of lipid hydroperoxide. Curcumin remarkably suppressed inflammation by reducing levels of inflammatory cytokines, including tumor necrosis factor-α (TNF-α), nuclear factor-kappa B (NF-κB) and interleukin-6 (IL-6). It also inhibited hepatic stellate cells (HSCs) activation by elevating the level of PPARγ and reducing the abundance of transforming growth factor-β (TGF-β). We found that oral administration of curcumin at 200 m |
| Disciplines: | Medicina |
| Keyword: | Gastroenterología, Medicina experimental, Farmacología, Fibrosis hepática, Estrés oxidativo, Curcumina, Factores de transcripción, Ratas |
| Keyword: | Gastroenterology, Experimental medicine, Pharmacology, Liver fibrosis, Oxidative stress, Curcumin, Transcription factors, Rats |
| Full text: | Texto completo (Ver PDF) |
