Validation of a liquid chromatography ultraviolet method for determination of herbicide diuron and its metabolites in soil samples
Document title: Validation of a liquid chromatography ultraviolet method for determination of herbicide diuron and its metabolites in soil samples
Journal: Anais da Academia Brasileira de Ciencias
Database: PERIÓDICA
System number: 000400420
ISSN: 0001-3765
Authors: 1
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Institutions: 1Universidade Estadual de Londrina, Departamento de Quimica, Londrina, Parana. Brasil
Year:
Season: Sep
Volumen: 88
Number: 3
Pages: 1235-1242
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Experimental, aplicado
English abstract Diuron is one of the most widely herbicide used worldwide, which can undergo degradation producing three primary metabolites: 3,4-dichlorophenylurea, 3-(3,4-dichlorophenyl)-1-methylurea, and 3,4-dichloroaniline. Since the persistence of diuron and its by-products in ecosystems involves risk of toxicity to environment and human health, a reliable quantitative method for simultaneous monitoring of these compounds is required. Hence, a simple method without preconcentration step was validated for quantitation of diuron and its main metabolites by high performance liquid chromatography with ultraviolet detection. Separation was achieved in less than 11 minutes using a C18 column, mobile phase composed of acetonitrile and water (45:55 v/v) at 0.86 mL min-1 and detection at 254 nm. The validated method using solid-liquid extraction followed by an isocratic chromatographic elution proved to be specific, precise and linear (R2 ˃ 0.99), presenting more than 90% of recovery. The method was successfully applied to quantify diuron and their by-products in soil samples collected in a sugarcane cultivation area, focusing on the environmental control
Disciplines: Química,
Agrociencias
Keyword: Química analítica,
Herbicidas,
Contaminación del suelo,
Química ambiental,
Cromatografía líquida,
Diuron,
Monitoreo ambiental
Keyword: Chemistry,
Agricultural sciences,
Analytical chemistry,
Herbicides,
Soil pollution,
Environmental chemistry,
Liquid chromatography,
Diuron,
Environmental screening
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