Urease activity according to the different inhibitors



Document title: Urease activity according to the different inhibitors
Journal: Anais da Academia Brasileira de Ciencias
Database: PERIÓDICA
System number: 000435712
ISSN: 0001-3765
Authors: 1
2
2
3
Institutions: 1Universidade Federal de Vicosa, Departamento de Solos, Vicosa, Minas Gerais. Brasil
2Universidade Federal Rural do Rio de Janeiro, Departamento de Solos, Seropedica, Rio de Janeiro. Brasil
3Universidade Federal de Vicosa, Departamento de Fitotecnia, Vicosa, Minas Gerais. Brasil
Year:
Season: Dic
Volumen: 90
Number: 4
Pages: 3685-3692
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Analítico, descriptivo
English abstract he objective of this study was to evaluate NBPT, zeolite and humic acid in different concentrations as inhibitors of the activity of the enzyme urease. The activity of the urease enzyme was quantified by the methods proposed by May and Douglas (1976) and Witte and Medina-Escobar (2001). For this reason, two experiments were conducted in a completely randomized design in split plots, in which the incubation periods are the plots and the fertilizers with the inhibitors at the different concentrations are the subplots with three replications. Fifteen fertilizers based on NBPT-coated urea, humic acid and zeolite were used in different concentrations. The two methods of analysis used in the experiment confirmed the efficiency of NBPT in inhibiting the enzymatic action even at low concentration, with 0.02% (m/m) being sufficient to inhibit urease, with no difference between inhibitor concentrations. The addition of humic acid reduced the activity of urease after 36 hours of reaction when the activity of the enzyme was evaluated by the method proposed by May and Douglas (1976). Zeolite did not influence the activity of the urease enzyme when analyzed by the May and Douglas method (1976)
Disciplines: Agrociencias
Keyword: Suelos,
Nutrición mineral,
Zeolitas,
Acidos húmicos,
Ureasa,
Actividad enzimática
Keyword: Soils,
Mineral nutrition,
Zeolites,
Humic acids,
Urease,
Enzymatic activity
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