| Journal: | Anais da Academia Brasileira de Ciencias |
| Database: | PERIÓDICA |
| System number: | 000420582 |
| ISSN: | 0001-3765 |
| Authors: | Rech, Virginia C1 Mezzomo, Nathana J2 Athaydes, Genaro A1 Feksa, Luciane R1 Figueiredo, Vandre C1 Kessler, Adriana1 Franceschi, Itiane D. de1 Wannmacher, Clovis M.D1 |
| Institutions: | 1Universidade Federal do Rio Grande do Sul, Instituto de Ciencias Basicas da Saude, Porto Alegre, Rio Grande do Sul. Brasil 2Centro Universitario Franciscano, Laboratorio de Nanotecnologia, Santa Maria, Rio Grande do Sul. Brasil |
| Year: | 2018 |
| Season: | Mar |
| Volumen: | 90 |
| Number: | 1 |
| Pages: | 99-108 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Analítico, descriptivo |
| English abstract | Considering that thiol-containing enzymes like kinases are critical for several metabolic pathways and energy homeostasis, we investigated the effects of cystine dimethyl ester and/or cysteamine administration on kinases crucial for energy metabolism in the kidney of Wistar rats. Animals were injected twice a day with 1.6 µmol/g body weight cystine dimethyl ester and/or 0.26 µmol/g body weight cysteamine from the 16th to the 20th postpartum day and euthanized after 12 hours. Pyruvate kinase, adenylate kinase, creatine kinase activities and thiol/disulfide ratio were determined. Cystine dimethyl ester administration reduced thiol/disulfide ratio and inhibited the kinases activities. Cysteamine administration increased the thiol/disulfide ratio and co-administration with cystine dimethyl ester prevented the inhibition of the enzymes. Regression between the thiol/disulfide ratio, and the kinases activities were significant. These results suggest that redox status may regulate energy metabolism in the rat kidney. If thiol-containing enzymes inhibition and oxidative stress occur in patients with cystinosis, it is possible that lysosomal cystine depletion may not be the only beneficial effect of cysteamine administration, but also its antioxidant and thiol-protector effect |
| Disciplines: | Biología |
| Keyword: | Fisiología animal, Mamíferos, Vías metabólicas, Homeostasis energética, Riñones, Cisteamina, Cinasas, Actividad enzimática, Ratas |
| Keyword: | Animal physiology, Mammals, Metabolic pathways, Energy homeostasis, Kidneys, Cysteamine, Kinases, Enzymatic activity, Rats |
| Full text: | Texto completo (Ver HTML) |
