| Journal: | Anais da Academia Brasileira de Ciencias |
| Database: | PERIÓDICA |
| System number: | 000412263 |
| ISSN: | 0001-3765 |
| Authors: | Martinelli, Arturo B1 Waichert, Cecilia1 Barbosa, Diego N1 Fagundes, Valeria1 Azevedo, Celso O1 |
| Institutions: | 1Universidade Federal do Espirito Santo, Departamento de Ciencias Biologicas, Vitoria, Espirito Santo. Brasil |
| Year: | 2017 |
| Season: | Sep |
| Volumen: | 89 |
| Number: | 3 |
| Pages: | 1629-1634 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | Genitalia are rich source of characters in insect taxonomy. Usually, they are examined after dissection and cleaning with potassium hydroxide (KOH), procedure that may damage both genital morphological structures and intracellular molecular contents. Enzymatic procedure with Proteinase K has been used to clean muscle off the genitalia while extract DNA, but its damage to the genital structures has not been evaluated. Herein, we qualitatively compare the use of KOH and Proteinase K to prepare genital structures in minute insects (Hymenoptera: Bethylidae). We show that Proteinase K is better to preserve the genital structure and provides quality DNA |
| Disciplines: | Biología |
| Keyword: | Anatomía e histología, Insectos, Avispas, Genitales, Técnicas histológicas, Proteinasas, Dissomphalus, Pristocera, Hymenoptera |
| Keyword: | Biology, Anatomy and histology, Insects, Wasps, Genitalia, Histological techniques, Proteinases, Dissomphalus, Pristocera, Hymenoptera |
| Full text: | Texto completo (Ver HTML) |
