Statistical tools application on dextranase production from Pochonia chlamydosporia (VC4) and its application on dextran removal from sugarcane juice



Document title: Statistical tools application on dextranase production from Pochonia chlamydosporia (VC4) and its application on dextran removal from sugarcane juice
Journal: Anais da Academia Brasileira de Ciencias
Database: PERIÓDICA
System number: 000420623
ISSN: 0001-3765
Authors: 1
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Institutions: 1Universidade Federal de Vicosa, Departamento de Bioquimica e Biologia Molecular, Vicosa, Minas Gerais. Brasil
2Universidade Federal de Vicosa, Departamento de Zootecnia, Vicosa, Minas Gerais. Brasil
3Universidade Vila Velha, Vila Velha, Espirito Santo. Brasil
4Universidade Federal de Vicosa, Departamento de Medicina Veterinaria, Vicosa, Minas Gerais. Brasil
Year:
Season: Mar
Volumen: 90
Number: 1
Pages: 461-470
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Analítico, descriptivo
English abstract The aim of this study was to optimize the dextranase production by fungus Pochonia chlamydosporia (VC4) and evaluate its activity in dextran reduction in sugarcane juice. The effects, over the P. chlamydosporia dextranase production, of different components from the culture medium were analyzed by Plackett-Burman design and central composite design. The response surface was utilized to determine the levels that, among the variables that influence dextranase production, provide higher production of these enzymes. The enzymatic effect on the removal of dextran present in sugarcane juice was also evaluated. It was observed that only NaNO3 and pH showed significant effect (p<0.05) over dextranase production and was determined that the levels which provided higher enzyme production were, respectively, 5 g/L and 5.5. The dextranases produced by fungus P. chlamydosporia reduced by 75% the dextran content of the sugarcane juice once treated for 12 hours, when compared to the control treatment
Disciplines: Química,
Biología
Keyword: Hongos,
Fermentaciones,
Biotecnología,
Jugo de caña,
Dextranasas,
Bioproducción,
Pochonia chlamydosporia
Keyword: Fungi,
Fermentation,
Biotechnology,
Sugar cane juice,
Dextranases,
Bioproduction,
Pochonia chlamydosporia
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