| Journal: | Anais da Academia Brasileira de Ciencias |
| Database: | PERIÓDICA |
| System number: | 000420616 |
| ISSN: | 0001-3765 |
| Authors: | Cipriano, Jamile L.D1 Cruz, Ana Claudia F2 Mancini, Karina C3 Schmildt, Edilson R3 Lopes, Jose Carlos4 Otoni, Wagner C2 Alexandre, Rodrigo S5 |
| Institutions: | 1Instituto Federal de Minas Gerais, Campus Sabara, Sabara, Minas Gerais. Brasil 2Universidade Federal de Vicosa, Departamento de Biologia Vegetal, Vicosa, Minas Gerais. Brasil 3Universidade Federal do Espirito Santo, Centro Universitario Norte do Espirito Santo, Sao Mateus, Espirito Santo. Brasil 4Universidade Federal do Espirito Santo, Departamento de Producao Vegetal, Alegre, Espirito Santo. Brasil 5Universidade Federal do Espirito Santo, Departamento de Ciencias Florestais e da Madeira, Jeronimo Monteiro, Espirito Santo. Brasil |
| Year: | 2018 |
| Season: | Mar |
| Volumen: | 90 |
| Number: | 1 |
| Pages: | 385-400 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Analítico, descriptivo |
| English abstract | The aim of this study was to evaluate somatic embryogenesis in juvenile explants of the THB papaya cultivar. Apical shoots and cotyledonary leaves were inoculated in an induction medium composed of different concentrations of 2,4-D (6, 9, 12, 15 and 18 µM) or 4-CPA (19, 22, 25, 28 and 31 µM). The embryogenic calluses were transferred to a maturation medium for 30 days. Histological analysis were done during the induction and scanning electron microscopy after maturing. For both types of auxin, embryogenesis was achieved at higher frequencies with cotyledonary leaves incubated in induction medium than with apical shoots; except for callogenesis. The early-stage embryos (e.g., globular or heart-shape) predominated. Among the auxins, best results were observed in cotyledonary leaves induced with 4-CPA (25 µM). Histological analyses of the cotyledonary leaf-derived calluses confirmed that the somatic embryos (SEs) formed from parenchyma cells, predominantly differentiated via indirect and multicellular origin and infrequently via synchronized embryogenesis. The secondary embryogenesis was observed during induction and maturation phases in papaya THB cultivar. The combination of ABA (0.5 µM) and AC (15 g L-1) in maturation medium resulted in the highest somatic embryogenesis induction frequency (70 SEs callus-1) and the lowest percentage of early germination (4%) |
| Disciplines: | Agrociencias |
| Keyword: | Frutales, Fisiología vegetal, Papaya, Cultivo de tejidos, Auxinas, Embriogénesis somática, Carica papaya |
| Keyword: | Fruit trees, Plant physiology, Papaya, Tissue culture, Auxins, Somatic embryogenesis, Carica papaya |
| Full text: | Texto completo (Ver HTML) |
