Reduction of the DNA damages, Hepatoprotective Effect and Antioxidant Potential of the Coconut Water, ascorbic and Caffeic Acids in Oxidative Stress Mediated by Ethanol
Document title: Reduction of the DNA damages, Hepatoprotective Effect and Antioxidant Potential of the Coconut Water, ascorbic and Caffeic Acids in Oxidative Stress Mediated by Ethanol
Journal: Anais da Academia Brasileira de Ciencias
Database: PERIÓDICA
System number: 000409013
ISSN: 0001-3765
Authors: 1
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2
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Institutions: 1Universidade de Sao Paulo, Instituto de Quimica, Sao Paulo. Brasil
2Universidade Federal de Sergipe, Departamento de Fisiologia, Sao Cristovao, Sergipe. Brasil
Year:
Season: Jun
Volumen: 89
Number: 2
Pages: 1095-1110
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Experimental, aplicado
English abstract Hepatic disorders such as steatosis and alcoholic steatohepatitis are common diseases that affect thousands of people around the globe. This study aims to identify the main phenol compounds using a new HPLC-ESI+-MS/MS method, to evaluate some oxidative stress parameters and the hepatoprotective action of green dwarf coconut water, caffeic and ascorbic acids on the liver and serum of rats treated with ethanol. The results showed five polyphenols in the lyophilized coconut water spiked with standards: chlorogenic acid (0.18 µM), caffeic acid (1.1 µM), methyl caffeate (0.03 µM), quercetin (0.08 µM) and ferulic acid (0.02 µM) isomers. In the animals, the activity of the serum γ-glutamyltranspeptidase (γ-GT) was reduced to 1.8 I.U/L in the coconut water group, 3.6 I.U/L in the ascorbic acid group and 2.9 I.U/L in the caffeic acid groups, when compared with the ethanol group (5.1 I.U/L, p<0.05). Still in liver, the DNA analysis demonstrated a decrease of oxidized bases compared to ethanol group of 36.2% and 48.0% for pretreated and post treated coconut water group respectively, 42.5% for the caffeic acid group, and 34.5% for the ascorbic acid group. The ascorbic acid was efficient in inhibiting the thiobarbituric acid reactive substances (TBARS) in the liver by 16.5% in comparison with the ethanol group. These data indicate that the green dwarf coconut water, caffeic and ascorbic acids have antioxidant, hepatoprotective and reduced DNA damage properties, thus decreasing the oxidative stress induced by ethanol metabolism
Disciplines: Química,
Medicina
Keyword: Bioquímica,
Estrés oxidativo,
Antioxidantes,
Compuestos fenólicos,
Agua de coco,
Acido ascórbico,
Acido cafeico
Keyword: Chemistry,
Medicine,
Biochemistry,
Oxidative stress,
Antioxidants,
Phenolic compounds,
Coconut water,
Ascorbic acid,
Caffeic acid
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