| Journal: | Anais da Academia Brasileira de Ciencias |
| Database: | PERIÓDICA |
| System number: | 000418008 |
| ISSN: | 0001-3765 |
| Authors: | Golunski, Simone1 Silva, Marceli F1 Marques, Camila T3 Rosseto, Vanusa3 Kaizer, Rosilene R3 Treichel, Helen3 Mossi, Altemir J3 Rigo, Diane1 Dallago, Rogerio M1 Luccio, Marco di2 |
| Institutions: | 1Universidade Regional Integrada do Alto Uruguai e das Missoes, Departamento de Engenharia de Alimentos, Erechim, Rio Grande do Sul. Brasil 2Universidade Federal de Santa Catarina, Departamento de Engenharia Quimica e de Alimentos, Florianopolis, Santa Catarina. Brasil 3Universidade Federal da Fronteira Sul, Programa de Pos-Graduacao em Ciencia e Tecnologia Ambiental, Erechim, Rio Grande do Sul. Brasil |
| Year: | 2017 |
| Season: | Mar |
| Volumen: | 89 |
| Number: | 1 |
| Pages: | 57-64 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | The present study evaluated the purification of inulinase by changing the ionic strength of the medium by addition of NaCl and CaCl2 followed by precipitation with n-propyl alcohol or iso-propyl alcohol. The effects of the concentration of alcohols and the rate of addition of alcohols in the crude extract on the purification yield and purification factor were evaluated. Precipitation caused an activation of enzyme and allowed purification factors up to 2.4-fold for both alcohols. The purification factor was affected positively by the modification of the ionic strength of the medium to 0.5 mol.L-1 NaCl before precipitation with the alcohol (n-propyl or iso-propyl). A purification factor of 4.8-fold and an enzyme yield of 78.1 % could be achieved by the addition of 0.5 mol.L-1 of NaCl to the crude extract, followed by the precipitation with 50 % (v/v) of n-propyl alcohol, added at a flow rate of 19.9 mL/min |
| Disciplines: | Química |
| Keyword: | Fitoquímica, Inulinasa, Extracción química, Precipitación, Fuerza iónica |
| Keyword: | Phytochemistry, Inulinase, Chemical extraction, Precipitation, Ionic strength |
| Full text: | Texto completo (Ver HTML) |
