| Journal: | Anais da Academia Brasileira de Ciencias |
| Database: | PERIÓDICA |
| System number: | 000436188 |
| ISSN: | 0001-3765 |
| Authors: | Gecibesler, Ibrahim Halil1 Aydin, Murat2 |
| Institutions: | 1Bingol University, Faculty of Health Sciences, Bingol. Turquía 2Bingol University, Faculty of Science and Art, Bingol. Turquía |
| Year: | 2020 |
| Volumen: | 92 |
| Number: | 1 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | Herbal-flavonoids (HF) as polyphenolic secondary metabolites are taken in the daily diet to join in many metabolic processes in the human organism. Anti-proliferative activities and human serum albumin (HSA) binding capacities of herbal-flavonoids namely 7,5’-dimethoxyisoetin (HF1), homoorientin-6’’-4-O-methyl-myo-inositol (HF2), (2R, 3R)-(+)-dihydrokaempferol-7,4’-dimethylether (HF3), eriodictyol-7,4’-dimethylether (HF4) and flavonoids isoorientin (HF5) and genkwanin (HF6) were investigated. Anti-proliferative activities were determined by the xCELLigence system by treatment with human prostate (PC3) and cervical cancer (HeLa) cells. The binding capacities were studied by two-dimensional (2D-FL) and three-dimensional (3D-FL) fluorescence spectroscopy. HeLa and PC3 cell lines were treated with flavonoids at 10, 50 and 100 μg/mL concentrations over a 48 hour period. Stable anti-proliferative efficacy plots were obtained for tested flavonoids. From the flavonoids, HF3 and HF4 showed the strongest anti-proliferative effect against PC3 and HeLa cell line. HF1 and HF2 exhibited the strongest binding capacity to the HSA corresponding to Kb values of 3.81 x 104 M-1 and 6.00 x 104 M-1, respectively. The studies revealed that the flavonoids form the basis of in vivo preclinical studies as important nutraceuticals of the daily diet, as well as modelled in medical and pharmacological applications |
| Disciplines: | Medicina |
| Keyword: | Farmacología, Oncología, Flavonoides, Albúmina sérica humana, Actividad antiproliferativa, Células cancerosas |
| Keyword: | Pharmacology, Oncology, Flavonoids, Human serum albumin, Antiproliferative activity, Cancer cells |
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