| Journal: | Anais da Academia Brasileira de Ciencias |
| Database: | PERIÓDICA |
| System number: | 000435845 |
| ISSN: | 0001-3765 |
| Authors: | Matos Rodrigues, Gabriel E1 Masseron, Carolinne C1 Silva, Fabio J. Moreira da1 Frajblat, Marcel1 Moreira, Lilian O1 Martins, Rodrigo A.P1 |
| Institutions: | 1Universidade Federal do Rio de Janeiro, Centro de Ciencias de Saude, Rio de Janeiro. Brasil |
| Year: | 2020 |
| Volumen: | 92 |
| Number: | 4 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Aplicado, descriptivo |
| English abstract | Pathogenic microbial detection and control in laboratory animal facilities is essential to guarantee animal welfare, data validity and reproducibility. Helicobacter spp. are known to affect mice health, what may interfere with experimental outcomes. This study aimed to screen for Helicobacter spp. in mice from animal facilities in Rio de Janeiro, Brazil using a PCR-based method. Primers designed to specifically identify Helicobacter spp. were used to amplify feces or intestine DNA extracted of mice from four different animal facilities. The expected 375 base pairs (bp) amplicon was purified, sequenced and a similarity of 95% was observed when compared to deposited sequences of H. hepaticus and H. bilis. In our screening, Helicobacter spp. was detected in ~59% of fecal and ~70% of intestine samples. Our study is the first to screen for Helicobacter spp. in mouse facilities of a Rio de Janeiro University using a low cost, rapid molecular diagnostic test. Although Helicobacter spp. screening is not mandatory according to Brazilian animal welfare regulation it is recommended by institutional animal health monitoring programs guidelines worldwide, including ARRIVE, AAALAC and FELASA |
| Disciplines: | Medicina veterinaria y zootecnia |
| Keyword: | Microbiología, Diagnóstico, Técnicas de diagnóstico, Reacción en cadena de la polimerasa (PCR), Helicobacter, Bioterio, Ratones |
| Keyword: | Microbiology, Diagnosis, Diagnostic techniques, Polymerase chain reaction (PCR), Helicobacter, Bioterium, Mice |
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