Modification of the Alere Giardia Ag TEST immunochromatography KIT methodology for its use in frozen fecal sediment of dogs and cats
Document title: Modification of the Alere Giardia Ag TEST immunochromatography KIT methodology for its use in frozen fecal sediment of dogs and cats
Journal: Anais da Academia Brasileira de Ciencias
Database: PERIÓDICA
System number: 000420625
ISSN: 0001-3765
Authors: 1
1
2
1
Institutions: 1Universidade Federal Fluminense, Instituto Biomedico, Niteroi, Rio de Janeiro. Brasil
2Universidade Federal Fluminense, Instituto de Matematica e Estatistica, Niteroi, Rio de Janeiro. Brasil
Year:
Season: Mar
Volumen: 90
Number: 1
Pages: 479-484
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Analítico, descriptivo
English abstract Giardia duodenalis is a worldwide intestinal parasite and is one of the most frequent protozoa species infecting dogs and cats. This study aimed to modify the methodology of Alere GIARDIA Ag TEST KIT for its use in frozen fecal sediments with different storage times in a freezer (-20°C), thus expanding the range of use of this methodology. One hundred fecal sediments from dogs (n=50) and cats (n=50) previously examined by optical microscopy for Giardia cysts were selected for this study. The agreement between the modified immunochromatography and microscopy results was calculated by Kappa coefficient. To evaluate the performance of the modified immunochromatography assay on samples with different storage time, the fecal sediments were divided into three groups according to the time of storage in a freezer: (a) ≤ 1 year (n=37); (b) > 1 year and ≤ 3 years (n=39); (c) > 10 years (max. 13 years) (n=24). The results obtained by the modified immunochromatography assay demonstrates a higher sensitivity of this technique when compared with microscopy, regardless of the frozen storage time. These results allow for the use of this methodology in a greater scope of analysis, especially in frozen fecal sediment triage in sample collections, enabling epidemiological and comparative analysis along different decades
Disciplines: Medicina veterinaria y zootecnia
Keyword: Pequeñas especies,
Parasitología,
Pruebas diagnósticas,
Inmunocromatografía,
Heces fecales,
Perros,
Gatos,
Giardia duodenalis
Keyword: Companion animals,
Parasitology,
Diagnostic tests,
Immunochromatography,
Fecal feces,
Cats,
Dogs,
Giardia duodenalis
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