Magnetic Parkia pendula seed gum as matrix for Concanavalin A lectin immobilization and its application in affinity purification
Document title: Magnetic Parkia pendula seed gum as matrix for Concanavalin A lectin immobilization and its application in affinity purification
Journal: Anais da Academia Brasileira de Ciencias
Database: PERIÓDICA
System number: 000402197
ISSN: 0001-3765
Authors: 1
2
1
1
1
Institutions: 1Universidade Federal de Pernambuco, Laboratorio de Imunopatologia Keizo Asami, Recife, Pernambuco. Brasil
2Universidade de Pernambuco, Faculdade de Ciencias, Educacao e Tecnologia de Garanhuns, Garanhuns, Pernambuco. Brasil
Year:
Season: Sep
Volumen: 86
Number: 3
Pages: 1351-1357
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Analítico, descriptivo
English abstract The present work aimed to magnetize Parkia pendula seeds gum and use it as a matrix for Concanavalin A covalent immobilization. This composite was applied in affinity purification of glycoconjugates. Parkia pendula seeds were hydrated and the gum provenient from the supernatant was precipitated and washed with ethanol and dried. The gum was magnetized in co-precipitation using solutions of Fe +2 and Fe +3 . Matrix activation was accomplished with NaIO 4 . Magnetized Parkia pendula seeds gum with covalently immobilized Concanavalin A was used as an affinity matrix for the recognition of bovine serum fetuin glycoprotein. Fetuin elution was carried out with a solution of glucose (300mM) and evaluated through SDS-PAGE. The efficiency of lectin immobilization and fetuin purification were 63% and 14%, respectively. These results indicate that the composite produced is a promising magnetic polysaccharide matrix for lectins immobilization. Thus, such system can be applied for affinity purification allowing an easy recovery by magnetic field
Disciplines: Física y astronomía,
Química
Keyword: Electromagnetismo,
Fitoquímica,
Química orgánica,
Inmovilización,
Magnetización,
Parkia pendula,
Semillas,
Concanavalina A
Keyword: Physics and astronomy,
Chemistry,
Electromagnetism,
Organic chemistry,
Phytochemistry,
Immobilization,
Magnetization,
Parkia pendula,
Seeds,
Concanavalin A
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