Lipase production by Aspergillus brasiliensis in solid-state cultivation of malt bagasse in different bioreactors configurations
Document title: Lipase production by Aspergillus brasiliensis in solid-state cultivation of malt bagasse in different bioreactors configurations
Journal: Anais da Academia Brasileira de Ciencias
Database: PERIÓDICA
System number: 000436036
ISSN: 0001-3765
Authors: 1
1
2
1
Institutions: 1Universidade Federal do Rio Grande do Sul, Laboratorio de Biotecnologia e Engenharia Bioquimica, Porto Alegre, Rio Grande do Sul. Brasil
2Universidade Estadual do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul. Brasil
Year:
Volumen: 92
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Experimental, aplicado
English abstract We evaluated the conditions to produce lipase in solid-state cultivation using a recently isolated strain of Aspergillus brasiliensis 157f in bioreactors of different configurations: static flat-bed, plugged-flow bed with forced water-saturated aeration, and pilot-scale rotating drum bioreactor, using malt bagasse as substrate. Lipase production was optimized applying experimental design analysis, which showed optima parameters defined as pH 7.7, addition of 11.3 % of soybean oil to the medium, and culture temperature of 32.7 oC, in static flat-bed. The highest enzyme activity (9.8 U.g-1 substrate) was obtained in the plugged-flow bed with forced water-saturated aeration. The fermented culture medium was lyophilized to create a solid enzymatic preparation (SEP), which was used to test the possibility of using this cheap biocatalyst in bioreactors to mediate esterification and transesterification reactions. SEP presented lipase activities of 7.35 U.g-1 substrate, indicating the possibility of further enhancing aspects of the use of such biocatalyst
Disciplines: Biología
Keyword: Hongos,
Biotecnología,
Cultivo en medio sólido,
Medios de cultivo,
Bagazo de malta,
Biorreactores,
Transesterificación,
Lipasas,
Aspergillus brasiliensis
Keyword: Fungi,
Biotechnology,
Solid media culture,
Culture media,
Brewery’s spent malt,
Bioreactors,
Transesterification,
Lipases,
Aspergillus brasiliensis
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