| Journal: | Anais da Academia Brasileira de Ciencias |
| Database: | PERIÓDICA |
| System number: | 000436073 |
| ISSN: | 0001-3765 |
| Authors: | D’Abadia, Patricia L1 Bailao, Elisa Flávia Luiz C1 Lino-Júnior, Ruy S2 Oliveira, Matheus Gabriel3 Silva, Vinicius B3 Oliveira, Leandra A.R4 Conceicao, Edemilson C4 Melo Reis, Paulo Roberto5 Borges, Leonardo Luiz1 Goncalves, Pablo José6 Almeida, Luciane M1 |
| Institutions: | 1Universidade Estadual de Goias, Campus Henrique Santillo, Anapolis, Goias. Brasil 2Universidade Federal de Goias, Instituto de Patologia Tropical e Saude Publica, Goiania, Goias. Brasil 3Pontificia Universidade Catolica de Goias, Escola de Ciencias Medicas, Goiania, Goias. Brasil 4Universidade Federal de Goias, Laboratorio de Pesquisa, Goiania, Goias. Brasil 5Pontificia Universidade Catolica de Goias, Laboratorio de Estudos Experimentais e Biotecnologicos, Goiania, Goias. Brasil 6Universidade Federal de Goias, Instituto de Fisica, Goiania, Goias. Brasil |
| Year: | 2020 |
| Volumen: | 92 |
| Number: | 2 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | The Hancornia speciosa latex reveals angiogenic, osteogenic, and anti-inflammatory properties, which present its potential for developing of wound healing drugs; however, the latex compounds responsible for angiogenesis remain unknown. One strategy to screen these active compounds is evaluation of latex fractions. This study aimed to obtain different fractions of latex and evaluate its angiogenic activity separately using the chick chorioallantoic membrane (CAM) assay. The serum (SE) fraction was responsible for angiogenesis, which was subject to biochemical characterization and computational simulations in order to understand the contribution of H. speciosa latex in wound healing process. Our results revealed weak antioxidant potential and absence of antimicrobial activity in the SE fraction. Phytochemical analysis identified chlorogenic acids (CGA) as the main compound of SE fraction. CGA bioactivity predictions identify different molecules associated with extracellular matrix (ECM) remodeling, such as metalloproteinases, which also are overexpressed in our CAM assay experiment. Docking simulations revealed the interactions between CGA and matrix metalloproteinase 2. In conclusion, SE latex fraction stimulates angiogenesis and may influence ECM remodeling. These properties may contribute to the wound healing process, and also confirm the widespread use of this plant |
| Disciplines: | Medicina |
| Keyword: | Farmacología, Heridas crónicas, Cicatrización, Metabolitos secundarios, Acido clorogénico, Látex, Angiogénesis, Hancornia speciosa, Apocynaceae |
| Keyword: | Pharmacology, Chronic wounds, Wound healing, Secondary metabolites, Chlorogenic acid, Latex, Angiogenesis, Hancornia speciosa, Apocynaceae |
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