Effects of cigarette smoke condensate on the production and characterization of exopolysaccharides by Bifidobacterium
Document title: Effects of cigarette smoke condensate on the production and characterization of exopolysaccharides by Bifidobacterium
Journal: Anais da Academia Brasileira de Ciencias
Database: PERIÓDICA
System number: 000401424
ISSN: 0001-3765
Authors: 1
1
1
2
1
Institutions: 1Zhengzhou University of Light Industry, College of Food and Biological Engineering, Zhengzhou, Henan. China
2Henan Normal University, College of Life Science, Xinxiang, Henan. China
Year:
Season: Jun
Volumen: 87
Number: 2
Pages: 997-1005
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Analítico, descriptivo
English abstract The aim of the study was to investigate the effect of cigarette smoke on the production and characterization of exopolysaccharides (EPSs) produced by Bifidobacterium. Cigarettes of Shanhua brand (nicotine: 1.1 mg, tar: 11 mg) were utilized to prepare a cigarette smoke condensate (CSC). The standard strain of Bifidobacterium animalis was cultured in MRS media under anaerobic addition of CSC. The results showed that CSC significantly decreased the growth of B. animalis as well as EPSs and acetic acid production. Furthermore, two EPSs fractions (Fr-I and Fr-II) were isolated and purified for chemical and molecular determination. By comparison with control, CSC was found to be of great impact on EPSs carbohydrate composition. The molecular weight mass of Fr-I changed from 3.33×105 g/mol (without CSC) to 2.99×105 (with CSC). In conclusion, in vitro studies revealed that CSC was directly able to affect the production of metabolites for B. animalis, which could be an essential factor in certain pathological disorders
Portuguese abstract O objetivo do estudo foi investigar o efeito da fumaça de cigarro sobre a produção e caracterização de exopolissacarídeos (EPSs) produzidos por Bifidobacterium. Os cigarros de marca Shanhua (nicotina: 1,1 mg, alcatrão: 11 mg) foram utilizados para preparar um condensado de fumaça de cigarro (CSC). A cepa padrão de Bifidobacterium animalis foi cultivada em meio MRS sob condições anaeróbicas do CSC. Os resultados mostraram que a CSC diminuiu significativamente o crescimento de B. animalis, bem como EPSs e produção de ácido acético. Além disso, duas frações de EPSs (Fr-I e Fr-II) foram isoladas e purificadas para determinação de componentes químicos e moleculares. Pela comparação com o controle, observou-se que o CSC apresentou uma grande diferença na composição de hidratos de carbono EPSs. A massa do peso molecular de Fr-I passou de 3,33 × 105 g / mol (sem CSC) para 2,99 × 105 (com CSC). Em conclusão, os estudos in vitro revelaram que a CSC foi capaz de afetar diretamente a produção de metabolitos de B. animalis, o que pode ser um fator essencial de certas desordens patológicas
Disciplines: Biología,
Química,
Medicina
Keyword: Bacterias,
Bioquímica,
Gastroenterología,
Exopolisacáridos,
Condensados,
Humo,
Cigarros,
Bifidobacterium,
Acido acético
Keyword: Biology,
Chemistry,
Medicine,
Bacteria,
Biochemistry,
Gastroenterology,
Exopolysaccharides,
Condensates,
Smoke,
Cigarettes,
Bifidobacterium,
Acetic acid
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