| Journal: | Anais da Academia Brasileira de Ciencias |
| Database: | PERIÓDICA |
| System number: | 000401303 |
| ISSN: | 0001-3765 |
| Authors: | Gohari, Ahmad Reza1 Mosaddegh, Mahmoud2 Naghibi, Farzaneh2 Eslami-Tehrani, Bahara2 Pirani, Atefeh2 Hamzeloo-Moghadam, Maryam3 Read, Roger W4 |
| Institutions: | 1Tehran University of Medical Sciences, Faculty of Pharmacy, Teherán. Irán 2Shahid Beheshti University of Medical Sciences, Traditional Medicine and Materia Medica Research Center, Teherán. Irán 3Shahid Beheshti University of Medical Sciences, School of Traditional Medicine, Teherán. Irán 4University of New South Wales, School of Chemistry, Sydney. Australia |
| Year: | 2015 |
| Season: | Jun |
| Volumen: | 87 |
| Number: | 2 |
| Pages: | 777-785 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Analítico, descriptivo |
| English abstract | Inula aucheriana DC is a member of the family Asteraceae which is known to produce cytotoxic secondary metabolites noted as sesquiterpene lactones. In the present study, sesquiterpene lactones inuchinenolide B, 6-deoxychamissonolide (stevin) and 14-acetoxy-1β,5α,7αH-4β-hydroxy-guai-9(10),11(13)-dien-12,8α- olide were isolated from I. aucheriana. Inuchinenolide B and 14-acetoxy-1β,5α,7αH-4β-hydroxy-guai- 9(10),11(13)-dien-12,8α-olide were further evaluated by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyl tetrazolium bromide) assay to demonstrate cytotoxic activity with IC50 values of (56.6, 19.0), (39.0, 11.8), and (55.7, 15.3) μg/mL against HepG-2, MCF-7 and A-549 cells, respectively. The cytotoxic activity of the two evaluated sesquiterpene lactones partly explains the cytotoxic activity that was previously observed for the extracts of Inula aucheriana. The isolated compounds could be further investigated in cancer research studies |
| Portuguese abstract | Inula aucheriana DC, uma espécie da família Asteraceae, é conhecida pela produção de lactonas sesquiterpênicas que são metabólitos secundários cititóxicos. No presente estudo, as seguintes lactonas sesquiterpênicas foram isoladas de I. aucheriana: inuchinenolida B, 6-desoxichamissonolida (estevina) e 14-acetoxi-1β,5α,7αH-4β-hidroxi-guai-9(10, 11(13)-dien- 12,8α-olida. A atividade citotóxica da inuchinenolida B e da 14-acetoxi-1β,5α,7αH-4β-hidroxi-guai-9(10),11(13)- dien-12,8α-olida foi avaliada pelo ensaio do MTT (brometo de 3-(4,5 dimetiltiazol-2il)-2,5- difeniltetrazólio) em culturas de células HepG-2, MCF-7 e A-549 tendo sido determinados valores de IC50 de (56.6, 19.0 μg/mL), (39.0, 11.8 μg/mL), e (55.7, 15.3 μg/ mL), respectivamente. A atividade citotóxica das duas lactonas sesquiterpênicas avaliadas explica a atividade citotóxica previamente observada para os extratos de Inula aucheriana. As substâncias isoladas merecem ser investigadas em estudos direcionados para pesquisas em câncer |
| Disciplines: | Biología, Química |
| Keyword: | Biología celular, Botánica, Fitoquímica, Citotoxicidad, Inula aucheriana, Bioensayos, Lactonas sesquiterpénicas |
| Keyword: | Biology, Chemistry, Botany, Cell biology, Phytochemistry, Cytotoxicity, Inula aucheriana, Bioassays, Sesquiterpene lactones |
| Full text: | Texto completo (Ver PDF) |
