| Journal: | Anais da Academia Brasileira de Ciencias |
| Database: | PERIÓDICA |
| System number: | 000400436 |
| ISSN: | 0001-3765 |
| Authors: | Carvalho, Francisco R.S1 Moura, Antonio G1 Rodrigues, Gardenia F1 Nunes, Narcia M.F2 Lima, Daisy J.B3 Costa, Marcilia P2 Ferreira, Paulo M.P2 Peron, Ana Paula1 |
| Institutions: | 1Universidade Federal do Piaui, Departamento de Ciencias Biologicas, Teresina, Piaui. Brasil 2Universidade Federal do Piaui, Programa de Pos-Graduacao em Ciencias Farmaceuticas, Teresina, Piaui. Brasil 3Universidade Federal do Ceara, Departamento de Fisiologia e Farmacologia, Fortaleza, Ceara. Brasil |
| Year: | 2016 |
| Season: | Sep |
| Volumen: | 88 |
| Number: | 3 |
| Pages: | 1419-1430 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | The objective of this study was to evaluate the antiproliferative, cytotoxic and genotoxic potential of salty liquid synthetic flavorings of Butter, Cheddar Cheese and Onion. The antiproliferative potential (2.9-1500 µg/mL) was assessed by MTT assay after 72h using the human tumor lines SF-295 (glioblastoma), OVCAR-8 (ovarian), HCT-116 (colon) and HL-60 (promyelocytic leukemia) and primary cultures of murine Sarcoma 180 (S180) and peripheral blood mononuclear cells (PBMC). Allium cepa bulbs were exposed to growing respective doses (1 mL and 2 mL). Only Butter and Cheddar flavorings revealed cytotoxic activity on cancer cells, with IC50 values ranging from 125.4 µg/mL (Cheddar - HCT-116) to 402.6 µg/mL (Butter - OVCAR-8). Butter flavoring was the most cytotoxic on PBMC (136.3 µg/mL) and increased cell division rate in relation to the mitotic index but did not cause cellular aberrations. Onion and Cheddar flavorings reduced the mitotic index after 24h and 48h exposure, but only Onion flavoring resulted in cellular aberrations and mitotic spindle abnormalities, such as anaphase and telophase bridges, micronucleated cells, conchicine-metaphases and amplifications. So, Butter, Onion and/or Cheddar flavorings caused significant changes in the division of meristematic cells of A. cepa and presented cytotoxic action even on decontrolled proliferating human tumor cells |
| Disciplines: | Medicina, Química |
| Keyword: | Oncología, Química farmacéutica, Aditivos alimenticios, Actividad antiproliferativa, Células neoplásicas, Saborizantes |
| Keyword: | Medicine, Chemistry, Oncology, Medicinal chemistry, Food additives, Antiproliferative activity, Neoplasic cells, Flavorings |
| Full text: | Texto completo (Ver HTML) |
