| Journal: | Acta scientiarum. Health science |
| Database: | PERIÓDICA |
| System number: | 000320062 |
| ISSN: | 1679-9291 |
| Authors: | Gasparino, Eliane1 Osorio, Paulo de Sa2 Soares, Maria Amelia Menck Marques, Debora Sommer Blanck, Danielly Veloso Luizetti, Fabiane |
| Institutions: | 1Universidade Estadual de Maringa, Departamento de Zootecnia, Maringa, Parana. Brasil 2Universidade Estadual do Oeste do Parana, Departamento de Biologia, Cascavel, Parana. Brasil |
| Year: | 2008 |
| Season: | Jul-Dic |
| Volumen: | 30 |
| Number: | 2 |
| Pages: | 101-106 |
| Country: | Brasil |
| Language: | Portugués |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | Under microscopic conditions, the invasive Entamoeba histolytica is indistinguishable from the non-pathogenic species Entamoeba dispar. In this way, the present study was carried out to determine a molecular strategy for discriminating both species by the mechanisms of bioinformatics. The gene cysteine synthetase was considered for such a purpose by using the resources of the National Center for Biotechnology Information data bank in the search for similarities in the gene sequence. In this way, a primer pair was designed by the Web Primer program and the restriction enzyme TaqI was selected by the Web Cutter software program. The DNA fragment had a size of 809 bp before cutting, which is consistent with E. dispar. The gene fragment was partitioned in a first fragment with 255 bp and a second one with 554 bp, which is similar to the genetic characteristics of E. histolytica |
| Portuguese abstract | Amebíase invasiva, causada por Entamoeba histolytica, é microscopicamente indistinguível da espécie não-patogênica Entamoeba dispar. Com auxílio de ferramentas de bioinformática, objetivou-se diferenciar Entamoeba histolytica e Entamoeba dispar por técnicas moleculares. A análise foi realizada a partir do banco de dados da National Center for Biotechnology Information; pela pesquisa de similaridade de sequências, elegeu-se o gene da cisteína sintase. Um par de primer foi desenhado (programa Web Primer) e foi selecionada a enzima de restrição TaqI (programa Web Cutter). Após a atuação da enzima, o fragmento foi dividido em dois, um com 255 pb e outro com 554 pb, padrão característico da E. histolytica. Na ausência de corte, o fragmento apresentou o tamanho de 809 pb, referente à E. dispar |
| Disciplines: | Biología, Química |
| Keyword: | Protozoarios, Taxonomía y sistemática, Bioquímica, Biotecnología, Biología molecular, Secuencia génica, Análisis de secuencias, Bioinformática, Entamoeba histolytica, Entamoeba dispar, Taxonomía molecular |
| Keyword: | Biology, Chemistry, Protozoa, Taxonomy and systematics, Biochemistry, Molecular biology, Gene sequence, Sequence analysis, Bioinformatics, Entamoeba histolytica, Entamoeba dispar, Molecular taxonomy |
| Full text: | Texto completo (Ver HTML) |
