Screening of whole yeast free-cells and optimization of pH and temperature for fructooligosaccharides production



Document title: Screening of whole yeast free-cells and optimization of pH and temperature for fructooligosaccharides production
Journal: Acta scientiarum. Biological sciences
Database: PERIÓDICA
System number: 000433739
ISSN: 1679-9283
Authors: 1
1
1
1
Institutions: 1Universidade Federal do Parana, Curitiba, Parana. Brasil
Year:
Season: Abr-Jun
Volumen: 39
Number: 2
Pages: 189-194
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Analítico, descriptivo
English abstract Fructooligosaccharides are catalyzed by β–fructofuranosidase enzyme, produced by many microorganisms. However, in order to achieve a more profitable, low time-consuming process with lower cost, researchers have sought alternatives. This study aimed to select and identify yeasts able to produce fructooligosaccharides and evaluate the influence of pH and temperature on their synthesis. Yeast suspensions, solutions of 500 g L-1 sucrose and three values of pH (4.5, 5.5, and 6.5) and temperature (40, 50, and 60ºC) were tested. Yeast species were identified by molecular techniques. Among 141 yeast isolates from grapes, 65 were able to synthesize fructooligosaccharides. The maximum concentration of fructooligosaccharides was 4.8% (w v-1), and Saccharomyces cerevisiae 222 produced 1-kestose and nystose
Portuguese abstract Fruto-oligossacarídeos são catalisados pelas enzimas β–fructofuranosidase, produzida por muitos micro-organismos. No entanto, para obter processos mais rentáveis, de menor custo e tempo, pesquisadores têm procurado alternativas. Este trabalho tem objetivo de selecionar e identificar leveduras capazes de produzir fruto-oligossacarídeos e avaliar a influência do pH e da temperatura na sua síntese. Suspensões de leveduras, soluções de sacarose de 500 g L-1 e três valores de pH (4,5, 5,5 e 6,5) e de temperaturas de (40, 50 e 60ºC) foram utilizados. As espécies de leveduras foram identificadas por técnicas moleculares. De 141 isolados de leveduras de uvas, 65 foram capazes de sintetizar fruto-oligossacarídeos. A concentração máxima de fruto-oligossacarídeos foi de 4,8% (p v-1), e a levedura Saccharomyces cerevisiae 222 produziu 1-cestose e nistose
Disciplines: Biología
Keyword: Biotecnología,
Levaduras,
Prebióticos,
Biotransformación,
Fructooligosácaridos,
Reacción en cadena de la polimerasa (PCR),
Saccharomyces cerevisiae
Keyword: Biotechnology,
Yeasts,
Prebiotics,
Biotransformation,
Fructooligosacharides,
Polymerase chain reaction (PCR),
Saccharomyces cerevisiae
Full text: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/34140/pdf