Experimental infection with different bacterial strains in larvae and juvenileLitopenaeus vannamei reared in Santa Catarina State, Brazil
Document title: Experimental infection with different bacterial strains in larvae and juvenileLitopenaeus vannamei reared in Santa Catarina State, Brazil
Journal: Acta scientiarum. Biological sciences
Database: PERIÓDICA
System number: 000334378
ISSN: 1679-9283
Authors: 1
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Institutions: 1Universidade Federal de Santa Catarina, Florianopolis, Santa Catarina. Brasil
Year:
Season: Jul-Sep
Volumen: 32
Number: 3
Pages: 291-296
Country: Brasil
Language: Portugués
Document type: Artículo
Approach: Experimental, analítico
English abstract This study evaluated the pathogenic characteristics of bacteria isolated from Litopenaeus vannamei during an outbreak at the Laboratory of Marine Shrimp, UFSC, Santa Catarina State, Brazil. Their virulence potential in larvae and juvenile shrimp and the effects on the total haemocyte count, phenoloxidase activity and serum agglutinate titre were examined after experimental infection. Bacterial strains were isolated from larvae and adult shrimps, identified by the AP120E biochemical system as: two strains of Vibrio alginolyticus, three of Aeromonas salmonicida and one of Pasteurella multocida sp. and Pasteurella sp. All the bacterial strains isolated in this study caused mortality in shrimp. One strain of V. alginolyticus was responsible for 97.3 and 88.7% mortality in larvae and juvenil shrimps, respectively. The shrimp immunological system was influenced by experimental infection with V. alginolyticus. Decrease in the total haemocyte count and increase in the phenoloxidase activity and the serum agglutinate titre (p < 0.05) were observed. The results showed the high pathogenicity of V. alginolyticus isolated from larvae and juvenile reared marine shrimp
Portuguese abstract Este estudo avaliou as características patogênicas de cepas de bactérias isoladas de Litopenaeus vannamei durante surto de mortalidade no Laboratório de Camarões Marinhos, UFSC, Estado de Santa Catarina, Brasil. Seu potencial de virulência em larvas e juvenis de camarão marinho e os efeitos sobre a contagem total de hemócito, atividade de fenoloxidase e título aglutinante do soro foram avaliados após infecção experimental. As cepas bacterianas foram isoladas de larvas e de camarões adultos e identificadas bioquimicamente pelo sistema API20E como: duas cepas de Vibrio alginolyticus, três de Aeromonas salmonicida e uma de Pasteurella sp. e P. multocida. Todas as cepas isoladas provocaram mortalidade em L. vannamei, e uma de V. alginolyticus resultou em mortalidade de 97,3 e 88,7% para larvas e juvenis de camarões, respectivamente. O sistema imunológico dos camarões juvenis sofreu influência da infecção experimental com V. alginolyticus, representado por redução significativa no número total de hemócitos circulantes e aumento na atividade da fenoloxidase e do título aglutinante do soro (p < 0,05). Os resultados deste ensaio demonstraram que a cepa de V. alginolyticus isolada apresentou alto poder de virulência para larvas e juvenis de camarão marinho cultivado
Disciplines: Biología
Keyword: Bacterias,
Crustáceos,
Inmunología,
Parasitología,
Camarón,
Vibrio alginolyticus,
Litopenaeus vannamei,
Infección experimental,
Ciclo de vida
Keyword: Biology,
Bacteria,
Crustaceans,
Immunology,
Parasitology,
Shrimp,
Vibrio alginolyticus,
Litopenaeus vannamei,
Experimental infection,
Life cycle
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