| Journal: | Acta scientiarum. Agronomy |
| Database: | PERIÓDICA |
| System number: | 000460110 |
| ISSN: | 1679-9275 |
| Authors: | Melo, Rita de Cássia Cerqueira1 Bragança, Carlos Augusto Dórea1 Pestana, Kátia Nogueira2 Silva, Harllen Sandro Alves da2 Ferreira, Cláudia Fortes2 Oliveira, Saulo Alves Santos de2 |
| Institutions: | 1Universidade Federal do Reconcavo da Bahia, Cruz das Almas, Bahia. Brasil 2Empresa Brasileira de Pesquisa Agropecuaria, Mandioca e Fruticultura, Cruz das Almas, Bahia. Brasil |
| Year: | 2019 |
| Volumen: | 41 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, analítico |
| English abstract | Modifications were made in the PCR conditions aiming to overcome the problem of non-amplification of the Xanthomonas phaseoli pv. manihotis (Xpm) fragment, using the primer pair XV / XK described in the literature. The objective of this study was to propose changes in the primers already described (XV / XK_MOD) and validate the use of these new primers in identifying Xpm. The validation procedure was carried out with the primer pair XV and XK_MOD, using different strains of Xpm, other plant pathogenic and endophytic bacteria genera and one isolate of X. phaseoli pv. passiflorae. As a control, additional reactions were conducted in multiplex with the universal primers for the 16S rRNA gene of the bacteria together with XV / XK and XV / XK_MOD. Using the forward primer (XV) described in the literature together with the modified reverse primer (XK_MOD), it was possible to achieve amplification from DNA extracted from in vitro cultures and from infected tissue, but no amplification was noticed for the primer pair described in the literature, confirming the effectiveness of the proposed modification |
| Disciplines: | Agrociencias, Biología |
| Keyword: | Plantas para uso industrial, Bacterias, Fitopatología, Yuca, Manihot esculenta, Xanthomonas phaseoli, Diagnóstico molecular, Resistencia |
| Keyword: | Plants for industrial use, Bacteria, Phytopathology, Cassava, Manihot esculenta, Xanthomonas phaseoli, Molecular diagnosis, Resistance |
| Full text: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
