| Journal: | Acta cirurgica brasileira |
| Database: | PERIÓDICA |
| System number: | 000271881 |
| ISSN: | 0102-8650 |
| Authors: | Oliveira, Alexandre Ferreira1 Castro e Silva, Tiago2 Sankarankutty, Ajith Kumar Pacheco, Eduardo Garcia Ferreira, Juliana Bagnato, Vanderlei Salvador Zucoloto, Sergio Castro e Silva, Orlando de |
| Institutions: | 1Universidade de Sao Paulo, Faculdade de Medicina, Ribeirao Preto, Sao Paulo. Brasil 2Universidade de Sao Paulo, Instituto de Fisica, Sao Carlos, Sao Paulo. Brasil |
| Year: | 2006 |
| Volumen: | 21 |
| Pages: | 29-32 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, analítico |
| English abstract | PURPOSE: To evaluate the effect of laser beam on remanescent liver after partial hepatectomy 90%. METHODS: Wistar rats, (N= 42), were divided into six groups with seven specimens each. The partial hepatectomy (HP) was performed in all animals through exeresis of approximately 90% of the liver parenchyma. The animals from groups HP and Laser application, HPL24, HPL48 and HPL72 undertook laser irradiation carried out through application (dose of 22.5 J/cm²) in five different sites in the remanescent liver. The rats were then sacrificed 24, 48 and 72hours after HP procedure, for the liver regeneration analysis,using the Proliferating Cell Nuclear Antigen (PCNA),and for dosage of serum aminotransferases. RESULTS: Were demonstrated an increase of the serum levels of alanine aminotransferase for the group of 24 hours and a decrease for the group of 72 hours exposed to laser. The index of marked cells had a considerable more improvement for the group of 72 hours exposed in laser compared to other groups. CONCLUSION: Laser did not cause hepatic injuries additional to the partial hepatectomy and perhaps led to a benefit by stimulating the proliferative activity |
| Portuguese abstract | OBJETIVO: Avaliar o efeito da luz laser em remanescentes hepáticos após hepatectomia a 90%. MÉTODOS: Aplicou-se luz laser com comprimento de onda de 660 nm e potência de 30 mw, por 30 segundos, na dose de 22,5 J/cm² em cada um deles, repetidos em cinco pontos do fígado remanescente, após realizada a hepatectomia a 90%. Os animais foram sacrificados para análise do fígado, contagem de hepatócitos em proliferação (Proliferating Cell Nuclear Antigen -PCNA), e coleta de sangue para dosagem serica das aminotransferases, após 24, 48 e 72 horas. RESULTADO: O índice de células marcadas teve significativo aumento no grupo de 72 horas irradiados com laser. CONCLUSÃO: A análise dos resultados demonstrou que o laser não causou lesões hepáticas adicionais à hepatectomia parcial e estimulou a atividade proliferativa hepatocelular |
| Disciplines: | Medicina |
| Keyword: | Cirugía, Gastroenterología, Terapéutica y rehabilitación, Regeneración hepática, Hígado, Láser, Insuficiencia hepática aguda, Hepatectomía parcial |
| Keyword: | Medicine, Gastroenterology, Surgery, Therapeutics and rehabilitation, Liver regeneration, Partial hepatectomy, Acute hepatic failure, Laser, Liver |
| Full text: | Texto completo (Ver HTML) |
