| Journal: | Acta cirurgica brasileira |
| Database: | PERIÓDICA |
| System number: | 000286157 |
| ISSN: | 0102-8650 |
| Authors: | Bakonyi-Neto, Alexandre1 Misiakos, Evangelos P Tzakis, Andreas G Behro, Mariana Ruiz, Phillip Miller, Joshua Ricordi, Camilo Takegawa, Bonifacio K |
| Institutions: | 1Universidade Estadual Paulista "Julio de Mesquita Filho", Faculdade de Medicina, Botucatu, Sao Paulo. Brasil |
| Year: | 2003 |
| Season: | Mar-Abr |
| Volumen: | 18 |
| Number: | 2 |
| Pages: | 81-85 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental |
| English abstract | Low cytoreductive regimen of irradiation associated to unmodified bone marrow infusion (UBM) does not prevent the occurrence of graft versus host disease (GVHD) after transplant. PURPOSE: In this study we evaluated the potential advantages of a long-term immunossupression and T-cell depleted bone marrow infusion (TCDBMI) in preventing the occurrence of GVHD after small bowel transplantation (SBTx). METHODS: Heterotopic SBTX was performed with Lewis rats as recipients and DA as donors and distributed into 5 groups according to the irradiation, duration of immunossupression and the use of UBM or TCDBMI: G1 (n=6), without irradiation and G2 (n=9), G3 (n=4), G4 (n=5) and G5 (n=6) was given 250 rd of irradiation. Groups 1,2,4 and G3 and 5 were infused with 100 x 10(6) UBM and TCDBM respectively. Animals in G1, 2, 3 were immunossupressed with 1mg/ FK506/Kg/IM for 5 days and G4 and G5 for 15 days. Anti CD3 monoclonal antibodies and immunomagnetic beads were used for T-cell depletion.Animals were examined for rejection, GVHD, chimerism characterization and ileal and skin biopsies. RESULTS: Minimal to mild rejection was observed in all groups; however, GVHD were present only in irradiated groups. Long-term immunossupression changed the severity of GVHD in G4 and G5. Rejection was the cause of death in G1 while GVHD in G2, 3, 4 and 5, not avoided by the use of TCDBMI. Total chimerism and T-cell chimerism was statistically higher in irradiated groups when compared to G1. CONCLUSION: Extended immunossupression associated to low dose of irradiation decrease the severity of GVHD, not avoided by the use of TCDBMI |
| Portuguese abstract | depletada |
| Disciplines: | Medicina |
| Keyword: | Cirugía, Medicina experimental, Inmunología, Trasplantes, Intestinos, Células T, Médula ósea, Depleción, Injerto-huésped |
| Keyword: | Medicine, Experimental medicine, Surgery, Intestines, Transplantation, T cells, Bone marrow, Depletion, Graft-host, Immunology |
| Full text: | Texto completo (Ver HTML) |
