Avaliação hormonal indireta e estudo da preservação folicular em tecido ovariano autólogo transplantado para região inguinal em ratos



Document title: Avaliação hormonal indireta e estudo da preservação folicular em tecido ovariano autólogo transplantado para região inguinal em ratos
Journal: Acta cirurgica brasileira
Database: PERIÓDICA
System number: 000286336
ISSN: 0102-8650
Authors: 1
1
2
Institutions: 1Universidade Federal do Parana, Curitiba, Parana. Brasil
2Pontificia Universidade Catolica do Parana, Curitiba, Parana. Brasil
Year:
Season: Ene-Feb
Volumen: 19
Number: 1
Pages: 27-30
Country: Brasil
Language: Portugués
Document type: Artículo
Approach: Experimental
English abstract PURPOSE: To analyse the effectivity technique of the subcutaneous autologous bisseccionated ovarian transplantation technique in inguinal region of the albine rats, close to the femoral vases, through the indirect study of hormonal production, made by the evaluation of the citology of the vaginal smears and the direct study of the tissue viability, carried out by histologic analysis. METHODS: 30 rats were analysed which suffered bilateral ooforectomy, being 15 of them intended for control and 15 of them suffered the ovarian transplantation. After 28 days, the grafting were recovered and evaluated. The analysis of the cytology of the vaginal smears were made in the moment 0, that is to say, pre-transplantation, and in the moment 1, post-transplantation, for all the animals and the histologic evaluation was accomplished at the moment 0 for the two groups and at the moment 1 for the animals that suffered transplantation. RESULTS: The results of the experiment demonstrated the viability and functionality of the grafts in 100% of the laminas evaluated, that were observed through the vaginal smears in estrous phase to all the rats of the experience group and by the presence of follicles in various stages of maturation, observed in the recovered grafts, indicating, directly, the viability of the ovarian tissue. CONCLUSION: Allowing to conclude that the ovarian transplantation is a viable alternative, in the conditions of the experiment, to the hormonal and follicular preservation in rats
Portuguese abstract ovariano. CONCLUSÃO: o transplante de tecido ovariano é uma alternativa viável, nas condições do experimento, para a preservação hormonal e folicular, em ratas
Disciplines: Medicina
Keyword: Cirugía,
Medicina experimental,
Estrógenos,
Progesterona,
Ovarios,
Trasplantes,
Hormonas
Keyword: Medicine,
Experimental medicine,
Surgery,
Estrogens,
Progesterone,
Ovaries,
Transplantation,
Hormones
Full text: Texto completo (Ver HTML)