| Journal: | Acta cirurgica brasileira |
| Database: | PERIÓDICA |
| System number: | 000285994 |
| ISSN: | 0102-8650 |
| Authors: | Jorge, Gracinda De Lourdes1 Leonardi, Luiz Sergio Boin, Ilka de Fatima Santana Ferreira Silva-Junior, Orlando de Castro e2 Escanhoela, Cecilia Amelia Fazzio |
| Institutions: | 1Universidade Estadual de Campinas, Faculdade de Ciencias Medicas, Campinas, Sao Paulo. Brasil 2Universidade de Sao Paulo, Faculdade de Medicina, Ribeirao Preto, Sao Paulo. Brasil |
| Year: | 2001 |
| Season: | Abr-Jun |
| Volumen: | 16 |
| Number: | 2 |
| Pages: | 75-81 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental |
| English abstract | The aim of this study was to describe a method for the induction of experimental secondary biliary fibrosis (SBF). Forty-seven Wistar rats were submitted to hepatic duct obstruction (OB group) for thirty days without ligature, section or cannulization causing interruption of biliary flow. This technique was carried out by simple traction of the bile duct passing it through the xiphoid appendix. Nine rats were submitted to a sham operation for bile duct stricture and seven rats comprised the control group. Blood samples were collected for the measurement of total bilirubin (TB), alkaline phosphatase (AP), alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Liver fragments were removed for morphological study. Thirty days after surgery TB, AP, ALT and AST levels were significantly increased in the hepatic duct ligation group compared to the sham operated group and the presence of SBF in the OB group was confirmed by morphological study of the liver. There was technical failure in 31.92% cases. The survival was 100% at fifteen days and 82.97% at the end of the experiment. We concluded that this simple surgical technique may be used to study the consequence of bile duct obstruction which could be a reversible process depending on the obstruction time. This technique can be carried out from cholestasis to fibrosis |
| Portuguese abstract | biliares em 81,2%, e moderada em 18,7% dos casos. Conseguiu-se desta maneira, o desenvolvimento de um modelo experimental alternativo que provoca a interrupção do fluxo bíleo-duodenal, semelhante à oclusão das vias biliares, sem necessidade de ligadura, canulação ou secção do ducto biliar. A obstrução biliar provocada pela tração do ducto biliar oferece excelentes condições de pesquisa desde o momento da interrupção do fluxo biliar até o desenvolvimento de colestase, acompanhada pela distorção da arquitetura hepática, caracterizada por fibrose portal com formação de septos e transformação nodular multi-focal |
| Disciplines: | Medicina |
| Keyword: | Cirugía, Medicina experimental, Cirrosis, Vías biliares, Obstrucción, Colestasis, Modelos experimentales |
| Keyword: | Medicine, Experimental medicine, Surgery, Cirrhosis, Cholestasis, Biliary tract, Obstruction, Experimental models |
| Full text: | Texto completo (Ver HTML) |
