Interaction between sucrose and pH during in vitro culture of Nephrolepis biserrata (Sw.) Schott (Pteridophyta)



Document title: Interaction between sucrose and pH during in vitro culture of Nephrolepis biserrata (Sw.) Schott (Pteridophyta)
Journal: Acta botanica brasilica
Database: PERIÓDICA
System number: 000271474
ISSN: 0102-3306
Authors: 1
Institutions: 1Empresa Brasileira de Pesquisa Agropecuaria, Semi-Arido, Petrolina, Pernambuco. Brasil
Year:
Season: Oct-Dic
Volumen: 18
Number: 4
Pages: 809-813
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Experimental, aplicado
English abstract The present work reports the effect of different pH and sucrose concentrations on in vitro propagation of Nephrolepis biserrata. Fronds aseptically obtained from stolon segment culture were cultivated in MS semi-solid medium supplemented with 0, 15, 30, 45 and 60g.L-1 sucrose and pH adjusted to 3, 5, 7 and 9, in a factorial design. Frond number and length, pinnae number, raquis length and diameter, fresh and dry matter weight were measured. Inhibition of shoot and leaf regeneration was observed in all the pH treatments in the absence of sucrose. On the other hand, when sucrose was added to the medium, the shoot number increased, reaching the maximum average values of 51.25 and 38.25 shoot per explant at pH 5 and 7, respectively. Sucrose concentrations from 15 to 45g.L-1 increased leaf length and diameter and the pH 9 did not affect the dry matter weight, and was still not adequate for development of new fronds. Young sporophytes were successfully acclimated
Portuguese abstract O presente trabalho relata o efeito de diferentes valores de pH e concentrações de sacarose na propagação in vitro de Nephrolepis biserrata. Frondes obtidas assepticamente a partir do cultivo de estolões foram inoculadas em meio semi-sólido MS, suplementado com 0, 15, 30, 45 e 60g.L-1 de sacarose e pH ajustado para 3, 5, 7 e 9, em arranjo fatorial. Foram avaliados: número e comprimento das frondes, número de pinas, comprimento e diâmetro da raque e biomassa fresca e seca da parte aérea. Em geral, houve inibição da regeneração de brotos e folhas em todos os tratamentos de pH na ausência de sacarose. Por outro lado, a presença de sacarose no meio induziu 51,25 e 38,25 brotos por explante no pH 5 e 7, respectivamente. Concentrações entre 15 e 45g.L-1 de sacarose aumentaram o comprimento e o diâmetro das folhas e o pH 9 não afetou o peso foliar seco, sendo inadequado para o desenvolvimento de novas frondes. Os esporófitos jovens foram submetidos, com sucesso, ao processo de aclimatação
Disciplines: Biología,
Agrociencias
Keyword: Briofitas y pteridofitas,
Fisiología vegetal,
Plantas ornamentales,
Helechos,
Propagación vegetativa,
Medios de cultivo,
pH,
Sacarosa,
Nephrolepis biserrata
Keyword: Biology,
Agricultural sciences,
Bryophytes and pteridophytes,
Plant physiology,
Ornamental plants,
Ferns,
Vegetative propagation,
Culture media,
pH,
Sucrose,
Nephrolepis biserrata
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