| Journal: | Archivos de cardiología de México |
| Database: | PERIÓDICA |
| System number: | 000343268 |
| ISSN: | 1405-9940 |
| Authors: | Ojeda Flores, Rafael1 Ortega López, Nayelli1 Avila Rodríguez, Miguel Angel1 Trejo Ballado, Fernando1 Alexánderson Rosas, Erick1 |
| Institutions: | 1Universidad Nacional Autónoma de México, México, Distrito Federal. México |
| Year: | 2010 |
| Season: | Oct-Dic |
| Volumen: | 80 |
| Number: | 4 |
| Pages: | 216-228 |
| Country: | México |
| Language: | Español |
| Document type: | Artículo |
| Approach: | Experimental, analítico |
| Spanish abstract | largo vertical y eje largo horizontal. Conclusiones: Se logró la estandarización de protocolos de adquisición de imágenes de los tres principales radiotrazadores utilizados para el estudio del metabolismo y perfusión cardiacos, en un modelo animal. Es factible establecer un protocolo válido para la valoración de perfusión, metabolismo glucolítico y oxidativo miocárdicos, con el fin de utilizarlo como punto de referencia para la evaluación de terapias génica, farmacológica o quirúrgica a nivel experimental |
| English abstract | Objective: To standardize an acquisition protocol for the study of myocardial glucolitic and oxidative metabolism and perfusion in a rat model. Methods: Studies were carried out with the three main radiopharmaceuticals used to assess heart function:[18F]–FDG for glucolitic metabolism; [1–11C]–acetate for oxidative metabolism and [13N]–NH3for myocardial perfusion.[18F]–FDG –Five Wistar adult male rats were studied in three different protocols: non–fasting group, fasting group,8 h before the study with water provided ad libitum, and a fasting group by the same time receiving an oral 50%–glucose solution. Thirty–minute scans were performed with a microPET Focus 120, 30 and 60 min after the administration of 370–555 MBq 18F–FDG. [1–11C]–Acetate –Eight rats were studied. Four static and four dynamic 30 min acquisitions after a 370–555 MBq of [1–11C]–acetate caudal vein administration.[13N]–NH3–Ten static studies were acquired 15 min post–administration of 370555 MBqof13NH3, under 1.5–2% isofluorane anesthesia. Comparative and visual analyses were performed by two experts in the field. A semi–quantitative analysis was performed using 3D reconstructions and ROI selections with AMIDE software. Results: The best images were those obtained from the non–fasting group, especially those taken at 60 min after the [18F]–FDG administration. High quality myocardial, static images were obtained with [1–11C]–acetate, and the dynamic acquisitions allowed the identification of myocardial perfusion. The 13NH3images showed a homogeneous distribution of the radiotracer in different segments of the short, long and horizontal axes in the left ventricle. Conclusions: It is possible to standardize the microPET acquisition protocols for the three main radiopharmaceuticals to evaluate the heart function in a rat model. It is feasible to establish a valid protocol for measuring glucolitic and oxidative myocardial metabolism and.. |
| Disciplines: | Medicina |
| Keyword: | Diagnóstico, Medicina experimental, Sistema cardiovascular, Radiotrazadores, Perfusión miocárdica, Metabolismo cardiaco, Miocardio, Imágenes, Modelos experimentales |
| Keyword: | Medicine, Cardiovascular system, Diagnosis, Experimental medicine, Radiotracers, Myocardial perfusion, Cardiac metabolism, Images, Experimental models |
| Full text: | Texto completo (Ver HTML) |
