| Revista: | Memorias do Instituto Oswaldo Cruz |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000459454 |
| ISSN: | 0074-0276 |
| Autores: | Moura, Alexandre S1 Costa da Silva, André Luis1 Peixoto, Pedro S2 Maciel, Ceres1 Cardoso, André F1 |
| Instituciones: | 1Universidade de Sao Paulo, Instituto de Ciencias Biomedicas, Sao Paulo. Brasil 2Universidade de Sao Paulo, Instituto de Matematica e Estatistica, Sao Paulo. Brasil |
| Año: | 2023 |
| Volumen: | 118 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | Culex quinquefasciatus, a cosmopolitan, domestic, and highly anthropophilic mosquito, is a vector of pathogenic arboviruses such as West Nile virus and Rift Valley virus, as well as lymphatic filariasis. The current knowledge on its reproductive physiology regarding vitellogenin expression in different tissues is still limited. OBJECTIVES In this study, we analysed the transcriptional profiles of vitellogenin genes in the fat body and ovaries of C. quinquefasciatus females during the first gonotrophic cycle. METHODS C. quinquefasciatus ovaries and/or fat bodies were dissected in different times during the first gonotrophic cycle and total RNA was extracted and used for reverse transcription polymerase chain reaction, quantitative real time-PCR, and in situ hybridisation. FINDINGS We confirmed the classical descriptions of the vitellogenic process in mosquitoes by verifying that vitellogenin genes are transcribed in the fat bodies of C. quinquefasciatus females. Using RNA in situ hybridisation approach, we showed that vitellogenin genes are also transcribed in developing ovaries, specifically by the follicle cells. MAIN CONCLUSIONS This is the first time that vitellogenin transcripts are observed in mosquito ovaries. Studies to determine if Vg transcripts are translated into proteins and their contribution to the reproductive success of the mosquito need to be further investigated |
| Disciplinas: | Biología |
| Palabras clave: | Insectos, Reproducción y desarrollo, Mosquitos, Fisiología reproductiva, Ovogénesis, Vitelogenina, Perfil de transcripción, Hibridación in situ, Culex quinquefasciatus |
| Keyword: | Insects, Reproduction and development, Mosquitoes, Reproductive physiology, Oogenesis, Vitellogenin, Transcription profile, In situ hybridization, Culex quinquefasciatus |
| Texto completo: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
