Vitellogenin genes are transcribed in Culex quinquefasciatus ovary



Título del documento: Vitellogenin genes are transcribed in Culex quinquefasciatus ovary
Revista: Memorias do Instituto Oswaldo Cruz
Base de datos: PERIÓDICA
Número de sistema: 000459454
ISSN: 0074-0276
Autores: 1
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2
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1
Instituciones: 1Universidade de Sao Paulo, Instituto de Ciencias Biomedicas, Sao Paulo. Brasil
2Universidade de Sao Paulo, Instituto de Matematica e Estatistica, Sao Paulo. Brasil
Año:
Volumen: 118
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés Culex quinquefasciatus, a cosmopolitan, domestic, and highly anthropophilic mosquito, is a vector of pathogenic arboviruses such as West Nile virus and Rift Valley virus, as well as lymphatic filariasis. The current knowledge on its reproductive physiology regarding vitellogenin expression in different tissues is still limited. OBJECTIVES In this study, we analysed the transcriptional profiles of vitellogenin genes in the fat body and ovaries of C. quinquefasciatus females during the first gonotrophic cycle. METHODS C. quinquefasciatus ovaries and/or fat bodies were dissected in different times during the first gonotrophic cycle and total RNA was extracted and used for reverse transcription polymerase chain reaction, quantitative real time-PCR, and in situ hybridisation. FINDINGS We confirmed the classical descriptions of the vitellogenic process in mosquitoes by verifying that vitellogenin genes are transcribed in the fat bodies of C. quinquefasciatus females. Using RNA in situ hybridisation approach, we showed that vitellogenin genes are also transcribed in developing ovaries, specifically by the follicle cells. MAIN CONCLUSIONS This is the first time that vitellogenin transcripts are observed in mosquito ovaries. Studies to determine if Vg transcripts are translated into proteins and their contribution to the reproductive success of the mosquito need to be further investigated
Disciplinas: Biología
Palabras clave: Insectos,
Reproducción y desarrollo,
Mosquitos,
Fisiología reproductiva,
Ovogénesis,
Vitelogenina,
Perfil de transcripción,
Hibridación in situ,
Culex quinquefasciatus
Keyword: Insects,
Reproduction and development,
Mosquitoes,
Reproductive physiology,
Oogenesis,
Vitellogenin,
Transcription profile,
In situ hybridization,
Culex quinquefasciatus
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