Polymorphic heterologous microsatellite loci for population genetics studies of the white-faced ibis Plegadis chihi (Vieillot, 1817) (Pelecaniformes, Threskiornithidae)



Título del documento: Polymorphic heterologous microsatellite loci for population genetics studies of the white-faced ibis Plegadis chihi (Vieillot, 1817) (Pelecaniformes, Threskiornithidae)
Revista: Genetics and molecular biology
Base de datos: PERIÓDICA
Número de sistema: 000343533
ISSN: 1415-4757
Autores: 1
1
1
Instituciones: 1Universidade Federal de Sao Carlos, Departamento de Genetica e Evolucao, Sao Carlos, Sao Paulo. Brasil
Año:
Periodo: Mar
Volumen: 35
Número: 1
Paginación: 74-80
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés We screened 44 heterologous microsatellites isolated in species of the families Threskiornithidae, Ciconiidae and Ardeidae for their use in a migratory waterbird, the white-faced ibis Plegadis chihi (Vieillot, 1817) (Threskiornithidae). Of the screened loci, 57% amplified successfully and 24% were polymorphic. In two breeding colonies from southern Brazil (N = 131) we detected 32 alleles (2-10 alleles/ locus). Average He over all loci and colonies was 0.55, and the combined probability of excluding false parents, 98%. There was no departure from HWE in any loci or population. Eru6 and Eru4 loci were in non-random association in the Alvorada colony, and NnNF5 and Eru5 in both populations. AMOVA analysis indicated that most of the genetic diversity was contained within populations. Structure analysis suggested a single population, and FST value showed weak genetic structuring (FST = 0.009, p = 0.05). The two populations are apparently connected through gene-flow. The panel of six microsatellites optimized here was sufficiently informative for characterizing the genetic diversity and structure in these natural populations of the white-faced ibis. The information generated could be useful in future studies of genetic diversity, relatedness and the mating system in Plegadis chihi and related species
Disciplinas: Biología
Palabras clave: Aves,
Genética,
Aves silvestres,
Genética de poblaciones,
Diversidad genética,
Marcadores moleculares,
Polimorfismo genético,
Plegadis chihi,
Pelecaniformes
Keyword: Biology,
Birds,
Genetics,
Wild birds,
Population genetics,
Genetic diversity,
Molecular markers,
Genetic polymorphism,
Plegadis chihi,
Pelecaniformes
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