Revista: | Genetics and molecular biology |
Base de datos: | PERIÓDICA |
Número de sistema: | 000343533 |
ISSN: | 1415-4757 |
Autores: | Souza, Andiara Silos Moraes de Castro e1 Miño, Carolina Isabel1 Lama, Silvia Nassif Del1 |
Instituciones: | 1Universidade Federal de Sao Carlos, Departamento de Genetica e Evolucao, Sao Carlos, Sao Paulo. Brasil |
Año: | 2012 |
Periodo: | Mar |
Volumen: | 35 |
Número: | 1 |
Paginación: | 74-80 |
País: | Brasil |
Idioma: | Inglés |
Tipo de documento: | Artículo |
Enfoque: | Experimental, aplicado |
Resumen en inglés | We screened 44 heterologous microsatellites isolated in species of the families Threskiornithidae, Ciconiidae and Ardeidae for their use in a migratory waterbird, the white-faced ibis Plegadis chihi (Vieillot, 1817) (Threskiornithidae). Of the screened loci, 57% amplified successfully and 24% were polymorphic. In two breeding colonies from southern Brazil (N = 131) we detected 32 alleles (2-10 alleles/ locus). Average He over all loci and colonies was 0.55, and the combined probability of excluding false parents, 98%. There was no departure from HWE in any loci or population. Eru6 and Eru4 loci were in non-random association in the Alvorada colony, and NnNF5 and Eru5 in both populations. AMOVA analysis indicated that most of the genetic diversity was contained within populations. Structure analysis suggested a single population, and FST value showed weak genetic structuring (FST = 0.009, p = 0.05). The two populations are apparently connected through gene-flow. The panel of six microsatellites optimized here was sufficiently informative for characterizing the genetic diversity and structure in these natural populations of the white-faced ibis. The information generated could be useful in future studies of genetic diversity, relatedness and the mating system in Plegadis chihi and related species |
Disciplinas: | Biología |
Palabras clave: | Aves, Genética, Aves silvestres, Genética de poblaciones, Diversidad genética, Marcadores moleculares, Polimorfismo genético, Plegadis chihi, Pelecaniformes |
Keyword: | Biology, Birds, Genetics, Wild birds, Population genetics, Genetic diversity, Molecular markers, Genetic polymorphism, Plegadis chihi, Pelecaniformes |
Texto completo: | Texto completo (Ver HTML) |