| Revista: | Electronic journal of biotechnology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000359606 |
| ISSN: | 0717-3458 |
| Autores: | Shan, Shi-hua1 Zhang, Ting-ting1 Li, Chun-juan1 Yang, Chen3 Yan, Cai-xia1 Wan, Shu-bo2 |
| Instituciones: | 1Shandong Peanut Research Institute, Quingdao, Shandong. China 2Shandong Academy of Agricultural Sciences, Jinan, Shandong. China 3Northeast Agricultural University, Harbin; Heilungkiang. China |
| Año: | 2011 |
| Volumen: | 14 |
| Número: | 6 |
| Paginación: | 6-6 |
| País: | Chile |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental |
| Resumen en inglés | Based on the conserved sequences of a known NBS resistance gene, a pair of degenerate primers was designed to amplify the NBS-LRR resistance gene from peanut using PCR and RACE methods. Results: Analyzing the amino acid sequence by BLAST on NCBI, which was deduced from the 1088bp-long gene named PnAG1-2, showed that it had a certain homology with some resistance proteins, among which Arachis cardenasii resistance protein gene had the highest homology (66%). Relative quantification PCR analysis indicated that PnAG1-2 gene expresses more in J11 (an A. flavus-resistant variety) than in JH1012 (an A. flavus-susceptible variety) when the harvest time was coming. Conclusions: In this study, the NBS-LRR resistance sequence was successfully cloned from peanut and prokaryotic expression was done on the gene, which provided a foundation for cultivating anti-A. flavus peanut varieties |
| Disciplinas: | Agrociencias, Química |
| Palabras clave: | Leguminosas, Bioquímica, Genética, Arachis cardenasii, Cacahuate, Bioinformática, NBS-LRR, Tiempo real de la fluorescencia, Reacción en cadena de la polimerasa (PCR) |
| Keyword: | Agricultural sciences, Chemistry, Legumes, Biochemistry, Genetics, Arachis cardenasii, Peanut, Bioinformatics, NBS-LRR, Real-time fluorescence, Polymerase chain reaction (PCR) |
| Texto completo: | Texto completo (Ver PDF) |
