Cloning and analysis of a NBS-LRR disease resistance gene candidate PnAG1 from peanut (Arachis hypogaea L.)
Título del documento: Cloning and analysis of a NBS-LRR disease resistance gene candidate PnAG1 from peanut (Arachis hypogaea L.)
Revista: Electronic journal of biotechnology
Base de datos: PERIÓDICA
Número de sistema: 000359606
ISSN: 0717-3458
Autors: 1
1
1
3
1
2
Institucions: 1Shandong Peanut Research Institute, Quingdao, Shandong. China
2Shandong Academy of Agricultural Sciences, Jinan, Shandong. China
3Northeast Agricultural University, Harbin; Heilungkiang. China
Any:
Volum: 14
Número: 6
Paginació: 6-6
País: Chile
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental
Resumen en inglés Based on the conserved sequences of a known NBS resistance gene, a pair of degenerate primers was designed to amplify the NBS-LRR resistance gene from peanut using PCR and RACE methods. Results: Analyzing the amino acid sequence by BLAST on NCBI, which was deduced from the 1088bp-long gene named PnAG1-2, showed that it had a certain homology with some resistance proteins, among which Arachis cardenasii resistance protein gene had the highest homology (66%). Relative quantification PCR analysis indicated that PnAG1-2 gene expresses more in J11 (an A. flavus-resistant variety) than in JH1012 (an A. flavus-susceptible variety) when the harvest time was coming. Conclusions: In this study, the NBS-LRR resistance sequence was successfully cloned from peanut and prokaryotic expression was done on the gene, which provided a foundation for cultivating anti-A. flavus peanut varieties
Disciplines Agrociencias,
Química
Paraules clau: Leguminosas,
Bioquímica,
Genética,
Arachis cardenasii,
Cacahuate,
Bioinformática,
NBS-LRR,
Tiempo real de la fluorescencia,
Reacción en cadena de la polimerasa (PCR)
Keyword: Agricultural sciences,
Chemistry,
Legumes,
Biochemistry,
Genetics,
Arachis cardenasii,
Peanut,
Bioinformatics,
NBS-LRR,
Real-time fluorescence,
Polymerase chain reaction (PCR)
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