Evaluation of the antigenotoxic and antioxidant activity induced by Croton antisyphiliticus



Título del documento: Evaluation of the antigenotoxic and antioxidant activity induced by Croton antisyphiliticus
Revista: Anais da Academia Brasileira de Ciencias
Base de datos: PERIÓDICA
Número de sistema: 000436009
ISSN: 0001-3765
Autores: 1
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Instituciones: 1Universidade Estadual Paulista "Julio de Mesquita Filho", Faculdade de Ciencias e Letras de Assis, Assis, Sao Paulo. Brasil
2Universidade Estadual Paulista "Julio de Mesquita Filho", Campu de Registro, Registro, Sao Paulo. Brasil
3Fundacao Educacional do Municipio de Assis, Assis, Sao Paulo. Brasil
Año:
Volumen: 92
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés This study aimed to investigate antigenotoxicity and antioxidant potential of extract, fractions and vitexin from C. antisyphiliticus. Methanolic extract was fractionated through solvents of increasing polarity. The composition of extracts and fractions were evaluated through phytochemical screening. Micronucleus test was performed in mice to evaluate the antigenotoxicity. Antioxidant activity was measured using the assay 1,1-diphenyl-2-picrylhydrazyl (DPPH), iron ion chelating, thiobarbituric acid assay and nitric oxide scavenging. Treatment with extract, fractions and vitexin did not produce an increase in Micronucleus mean values. However, Micronucleus (MN) mean values decreased in relation to control. methanolic extract presented antioxidant potential for DPPH (81%), iron ion chelating (77.8%), Thiobarbituric Acid (TBARS) (32.49%) and Nitric Oxide (NO) (80.97%). Ethyl acetate fraction showed the highest antioxidant activity (65.46%). The vitexin showed a Inhibitory Concentration (IC50) of DPPH value smaller in relation to control. Vitexin flavonoid was detected by High Performance Liquid Chromatography (HPLC), infrared spectrometry and nuclear magnetic resonance. It can be inferred that methanolic extract, fraction ethyl acetate and vitexin isolated from C. antisyphiliticus is endowed with antigenotoxic and antioxidant potential
Disciplinas: Química,
Biología
Palabras clave: Fitoquímica,
Angiospermas,
Plantas medicinales,
Compuestos fenólicos,
Flavonoides,
Antigenotoxicidad,
Actividad antioxidante,
Bioensayos,
Ratones,
Croton antisyphiliticus,
Euphorbiaceae
Keyword: Phytochemistry,
Angiosperms,
Medicinal plants,
Phenolic compounds,
Flavonoids,
Antigenotoxicity,
Antioxidant activity,
Bioassays,
Mice,
Croton antisyphiliticus,
Euphorbiaceae
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