First report of putative Leishmania RNA virus 2 (LRV2) in Leishmania infantum strains from canine and human visceral leishmaniasis cases in the southeast of Brazil



Título del documento: First report of putative Leishmania RNA virus 2 (LRV2) in Leishmania infantum strains from canine and human visceral leishmaniasis cases in the southeast of Brazil
Revista: Memorias do Instituto Oswaldo Cruz
Base de datos: PERIÓDICA
Número de sistema: 000459446
ISSN: 0074-0276
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Instituciones: 1Fundacao Oswaldo Cruz, Instituto Rene Rachou, Belo Horizonte, Minas Gerais. Brasil
2Universidade Federal de Sao Joao del Rei, Laboratorio de Doencas Parasitarias e Infecciosas, Divinopolis, Minas Gerais. Brasil
3Ministerio da Agricultura, Pecuaria e Abastecimento, Laboratorio Nacional Agropecuaria, Pedro Leopoldo, Minas Gerais. Brasil
4Universidade de Sao Paulo, Faculdade de Medicina, Sao Paulo. Brasil
5Universidade Federal da Bahia, Departamento de Anatomia, Patologia e Clinicas Veterinarias, Salvador, Bahia. Brasil
6Universidade Federal de Minas Gerais, Instituto de Ciencias Biologicas, Belo Horizonte, Minas Gerais. Brasil
7Universidade Federal do Parana, Departamento de Engenharia de Bioprocessos e Biotecnologia, Curitiba, Parana. Brasil
8University of Ostrava, Faculty of Science, Ostrava. República Checa
Año:
Volumen: 118
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Aplicado, descriptivo
Resumen en inglés Leishmania RNA virus 1 (LRV1) is commonly found in South American Leishmania parasites belonging to the subgenus Viannia, whereas Leishmania RNA virus 2 (LRV2) was previously thought to be restricted to the Old-World pathogens of the subgenus Leishmania. OBJECTIVES In this study, we investigated the presence of LRV2 in strains of Leishmania (L.) infantum, the causative agent of visceral leishmaniasis (VL), originating from different hosts, clinical forms, and geographical regions. METHODS A total of seventy-one isolates were screened for LRV2 using semi-nested reverse transcription-polymerase chain reaction (RT-PCR) targeting the RNA-dependent RNA polymerase (RdRp) gene. FINDINGS We detected LRV2 in two L. infantum isolates (CUR268 and HP-EMO) from canine and human cases, respectively. MAIN CONCLUSIONS To the best of our knowledge, this is the first detection of LRV2 in the New World
Disciplinas: Biología
Palabras clave: Protozoarios,
Virus,
Leishmaniasis,
Leishmania infantum,
Virus de ARN,
Totiviridae
Keyword: Protozoa,
Virus,
Leishmaniasis,
Leishmania infantum,
RNA virus,
Totiviridae
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