Transcriptome analysis reveals gene expression changes of pigs infected with non-lethal African swine fever virus



Título del documento: Transcriptome analysis reveals gene expression changes of pigs infected with non-lethal African swine fever virus
Revista: Genetics and molecular biology
Base de datos: PERIÓDICA
Número de sistema: 000459188
ISSN: 1415-4757
Autores: 1
1
1
2
2
1
1
1
Instituciones: 1State Key Laboratory of Animal Biotech Breeding, Beijing. China
2International Livestock Research Institute, Nairobi. Kenia
Año:
Volumen: 46
Número: 3
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Analítico, descriptivo
Resumen en inglés African swine fever (ASF) is an important viral disease of swine caused by the African swine fever virus (ASFV), which threatens swine production profoundly. To better understand the gene expression changes when pig infected with ASFV, RNA sequencing was performed to characterize differentially expressed genes (DEGs) of six tissues from Kenya domestic pigs and Landrace × Yorkshire (L/Y) pigs infected with ASFV Kenya1033 in vivo. As results, a total of 209, 522, 34, 505, 634 and 138 DEGs (q-value < 0.05 and |Log2foldchange| values >2) were detected in the kidney, liver, mesenteric lymph node, peripheral blood mononuclear cell, submandibular lymph node and spleen, respectively. The expression profiles of DEGs shared in the multiple tissues illustrated variation in regulation function in the different tissues. Functional annotation analysis and interaction of proteins encoded by DEGs revealed that genes including IFIT1, IFITM1, MX1, OASL, ISG15, SAMHD1, IFINA1, S100A12 and S100A8 enriched in the immune and antivirus pathways were significantly changed when the hosts were infected with ASFV. The genes mentioned could play crucial roles in the process of the reaction to non-lethal ASF infection, which may will help to improve the ASF tolerance in the pig population through molecular breeding strategies
Disciplinas: Biología
Palabras clave: Porcinos,
Virus,
Genética,
Peste porcina africana,
Transcriptoma,
Expresión génica
Keyword: Swine,
Virus,
Genetics,
Swine fever-african,
Transcriptome,
Gene expression
Texto completo: Texto completo (Ver HTML) Texto completo (Ver PDF)