| Revista: | Electronic journal of biotechnology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000336254 |
| ISSN: | 0717-3458 |
| Autores: | Wang, Chuan Tang1 Tang, Yue Yi1 Wang, Xiu Zhen1 Zhang, Shu Wei2 Li, Gui Jie1 Zhang, Jian Cheng1 Yu, Shan Lin1 |
| Instituciones: | 1Shandong Peanut Research Institute, Qingdao. China 2Shandong Agricultural University, Taian City, Shandong. China |
| Año: | 2011 |
| Periodo: | Mar |
| Volumen: | 14 |
| Número: | 2 |
| País: | Chile |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | Screening of peanut seeds resulting from 0.39% sodium azide treatment with NIRS calibration equation for bulk seed samples identified a plant with more than 60% oleate. Oleate content in individual seeds of the plant, as predicted by NIRS calibration equation for intact single peanut seeds, ranged from 50.05% ~ 68.69%. Three seeds with >60% oleate thus identified were further confirmed by gas chromatography. Multiple sequence alignments of the FAD2B gene from Huayu 22 (wild type) and peanut seeds with elevated oleate (mutant type) revealed a C281T transition in the coding region causing an I94T substitution in the oleoyl-PC desaturase, which may be responsible for reduction in the enzyme activity |
| Disciplinas: | Biología, Agrociencias |
| Palabras clave: | Frutales, Biotecnología, Cacahuate, Mutagénesis, Azida de sodio, Oleatos, Mejoramiento genético |
| Keyword: | Biology, Agricultural sciences, Fruit trees, Biotechnology, Peanut, Mutagenesis, Sodium azide, Oleates, Plant breeding |
| Texto completo: | Texto completo (Ver PDF) |
