Antitumor activity of C-phycocyanin from Arthronema africanum (Cyanophyceae)



Título del documento: Antitumor activity of C-phycocyanin from Arthronema africanum (Cyanophyceae)
Revista: Brazilian archives of biology and technology
Base de datos: PERIÓDICA
Número de sistema: 000388221
ISSN: 1516-8913
Autores: 1
1
1
2
2
2
Instituciones: 1Bulgarian Academy of Sciences, Institute of Experimental Morphology, Pathology and Anthropology with Museum, Sofía. Bulgaria
2Bulgarian Academy of Sciences, Institute of Plant Physiology and Genetics, Sofía. Bulgaria
Año:
Periodo: Sep-Oct
Volumen: 57
Número: 5
Paginación: 675-684
País: Brasil
Idioma: Inglés
Tipo de documento: Nota breve o noticia
Enfoque: Experimental
Resumen en inglés Pure C-phycocyanin (C-PC) was isolated from Arthronema africanumto evaluate its potential antitumor effects in vivo and in vitro. Experimental myeloid Graffi tumor in hamsters was used as a model. The cell proliferation assay showed that C-PC treatment, at concentration of 100 µg mL-1 for 24 h, significantly inhibited the growth of Graffi tumor cells (51.4% viability). Agarose gel electrophoresis of the genomic DNA of treated cells displayed time-and concentration-dependent fragmentation pattern, typical for apoptosis. Apoptotic process was related to the increase in cellular manganese and copper/zinc superoxide dismutases and glutathione reductase activities, coupled with a low catalase activity. In vivo C-PC administration (5.0 mg kg-1 body weight) suppressed the tumor transplantability and growth, while the mean survival time of the tumor-bearing hamsters was increased. The results revealed promising antitumor activities of A. africanum C-PC and suggested the potential of this natural biliprotein pigment for future pharmacological and medical applications. The study provided new data on the mechanism of the C-PC induced apoptosis in which the imbalance of antioxidant enzymes that favoured hydrogen peroxide accumulation might play a leading role
Disciplinas: Biología,
Medicina
Palabras clave: Algas,
Farmacología,
Medicina experimental,
Oncología,
Ficocianina-C,
Actividad antitumoral,
Tumor mieloide de Graffi,
Arthronema africanum,
Cyanophyceae
Keyword: Biology,
Medicine,
Algae,
Experimental medicine,
Oncology,
Pharmacology,
C-phycocyanin,
Antitumoral activity,
Graffi myeloid tumor,
Arthronema africanum,
Cyanophyceae
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