| Revista: | Brazilian archives of biology and technology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000388221 |
| ISSN: | 1516-8913 |
| Autors: | Gardeva, Elena1 Toshkova, Reneta1 Yossifova, Liliya1 Minkova, Kaledona2 Ivanova, Natalia2 Gigova, Liliana2 |
| Institucions: | 1Bulgarian Academy of Sciences, Institute of Experimental Morphology, Pathology and Anthropology with Museum, Sofía. Bulgaria 2Bulgarian Academy of Sciences, Institute of Plant Physiology and Genetics, Sofía. Bulgaria |
| Any: | 2014 |
| Període: | Sep-Oct |
| Volum: | 57 |
| Número: | 5 |
| Paginació: | 675-684 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Nota breve o noticia |
| Enfoque: | Experimental |
| Resumen en inglés | Pure C-phycocyanin (C-PC) was isolated from Arthronema africanumto evaluate its potential antitumor effects in vivo and in vitro. Experimental myeloid Graffi tumor in hamsters was used as a model. The cell proliferation assay showed that C-PC treatment, at concentration of 100 µg mL-1 for 24 h, significantly inhibited the growth of Graffi tumor cells (51.4% viability). Agarose gel electrophoresis of the genomic DNA of treated cells displayed time-and concentration-dependent fragmentation pattern, typical for apoptosis. Apoptotic process was related to the increase in cellular manganese and copper/zinc superoxide dismutases and glutathione reductase activities, coupled with a low catalase activity. In vivo C-PC administration (5.0 mg kg-1 body weight) suppressed the tumor transplantability and growth, while the mean survival time of the tumor-bearing hamsters was increased. The results revealed promising antitumor activities of A. africanum C-PC and suggested the potential of this natural biliprotein pigment for future pharmacological and medical applications. The study provided new data on the mechanism of the C-PC induced apoptosis in which the imbalance of antioxidant enzymes that favoured hydrogen peroxide accumulation might play a leading role |
| Disciplines | Biología, Medicina |
| Paraules clau: | Algas, Farmacología, Medicina experimental, Oncología, Ficocianina-C, Actividad antitumoral, Tumor mieloide de Graffi, Arthronema africanum, Cyanophyceae |
| Keyword: | Biology, Medicine, Algae, Experimental medicine, Oncology, Pharmacology, C-phycocyanin, Antitumoral activity, Graffi myeloid tumor, Arthronema africanum, Cyanophyceae |
| Text complet: | Texto completo (Ver HTML) |
