Geobacillus stearothermophilus LV cadA gene mediates resistance to cadmium, lead and zinc in zntA mutants of Salmonella enterica serovar Typhimurium



Título del documento: Geobacillus stearothermophilus LV cadA gene mediates resistance to cadmium, lead and zinc in zntA mutants of Salmonella enterica serovar Typhimurium
Revista: Biological research
Base de datos: PERIÓDICA
Número de sistema: 000269180
ISSN: 0716-9760
Autores: 1



2
Instituciones: 1Universidad de Santiago de Chile, Facultad de Química y Biología, Santiago de Chile. Chile
2Chiron Corporation, Blood Testing Division, Emeryville, California. Estados Unidos de América
Año:
Volumen: 39
Número: 4
Paginación: 661-668
País: Chile
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental
Resumen en inglés Salmonella entérica serovar Typhimurium cells expressing the cadA gene of Geobacillus stearothermophilus LV exhibit a hypersensitive phenotype to cadmium chloride. Deletion of the ORF STM3576 from the Salmonella genome resulted in cadmium, lead and zinc sensitivity, confirming that this ORF is a homologue of the zntA gene. The observed sensitivity was reverted upon expression of the G. stearothermophilus LV cadA gene. These results indicate that the cadA gene product is involved in Cd, Pb and Zn resistance as a classical P-type ATPase and strongly suggest that the observed hypersensitive phenotype to these metals can be related to the function of the host zntA gene product
Disciplinas: Biología,
Química
Palabras clave: Bacterias,
Genética,
Bioquímica,
Genes,
Geobacillus stearothermophilus,
Salmonella enterica,
Cadmio,
Plomo,
Zinc,
Gene ZntA
Keyword: Biology,
Chemistry,
Bacteria,
Genetics,
Biochemistry,
Genes,
Salmonella enterica,
Cadmium,
Lead,
Geobacillus stearothermophilus,
Zinc
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