Cloning and functional characterization of the gene encoding the transcription factor Ace 1 in the basidiomycete Phanerochaete chrysosporium



Título del documento: Cloning and functional characterization of the gene encoding the transcription factor Ace 1 in the basidiomycete Phanerochaete chrysosporium
Revista: Biological research
Base de datos: PERIÓDICA
Número de sistema: 000269175
ISSN: 0716-9760
Autores: 1



2
Instituciones: 1Pontificia Universidad Católica de Chile, Facultad de Ciencias Biológicas, Santiago de Chile. Chile
2Universidad de Chile, Facultad de Ciencias Químicas y Farmacéuticas, Santiago de Chile. Chile
Año:
Volumen: 39
Número: 4
Paginación: 641-648
País: Chile
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Analítico, descriptivo
Resumen en inglés In this report we describe the isolation and characterization of a gene encoding the transcription factor Acel (Activation protein of cup 1 Expression) in the white rot fungus Phanerochaete chrysosporium. Pc-acel encodes a predicted protein of 633 amino acids containing the copper-fist DNA binding domain typically found in fungal transcription factors such as Acel, Macl and Haal from Saccharomyces cerevisiae. The Pc-acel gene is localized in Scaffold 5, between coordinates 220841 and 222983. A S. cerevisiae acel null mutant strain unable to grow in high-copper medium was fully complemented by transformation with the cDNA of Pc-acel. Moreover, Northern blot hybridization studies indicated that Pc-acel cDNA restores copper inducibility of the yeast cup 1 gene, which encodes the metal-binding protein metallothionein implicated in copper resistance. To our knowledge, this is first report describing an Acel transcription factor in basidiomycetes
Disciplinas: Química,
Biología
Palabras clave: Bioquímica,
Genética,
Hongos,
Factores de transcripción,
Genes,
Basidiomycetes,
Phanerochaete chrysosporium
Keyword: Chemistry,
Biology,
Biochemistry,
Fungi,
Genetics,
Transcription factors,
Genes,
Basidiomycetes,
Phanerochaete chrysosporium
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