| Revista: | Biological research |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000269175 |
| ISSN: | 0716-9760 |
| Autors: | Polanco, Rubén1 Canessa, Paulo Rivas, Alexis Larrondo, Luis F Lobos, Sergio2 Vicuña, Rafael |
| Institucions: | 1Pontificia Universidad Católica de Chile, Facultad de Ciencias Biológicas, Santiago de Chile. Chile 2Universidad de Chile, Facultad de Ciencias Químicas y Farmacéuticas, Santiago de Chile. Chile |
| Any: | 2006 |
| Volum: | 39 |
| Número: | 4 |
| Paginació: | 641-648 |
| País: | Chile |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Analítico, descriptivo |
| Resumen en inglés | In this report we describe the isolation and characterization of a gene encoding the transcription factor Acel (Activation protein of cup 1 Expression) in the white rot fungus Phanerochaete chrysosporium. Pc-acel encodes a predicted protein of 633 amino acids containing the copper-fist DNA binding domain typically found in fungal transcription factors such as Acel, Macl and Haal from Saccharomyces cerevisiae. The Pc-acel gene is localized in Scaffold 5, between coordinates 220841 and 222983. A S. cerevisiae acel null mutant strain unable to grow in high-copper medium was fully complemented by transformation with the cDNA of Pc-acel. Moreover, Northern blot hybridization studies indicated that Pc-acel cDNA restores copper inducibility of the yeast cup 1 gene, which encodes the metal-binding protein metallothionein implicated in copper resistance. To our knowledge, this is first report describing an Acel transcription factor in basidiomycetes |
| Disciplines | Química, Biología |
| Paraules clau: | Bioquímica, Genética, Hongos, Factores de transcripción, Genes, Basidiomycetes, Phanerochaete chrysosporium |
| Keyword: | Chemistry, Biology, Biochemistry, Fungi, Genetics, Transcription factors, Genes, Basidiomycetes, Phanerochaete chrysosporium |
| Text complet: | Texto completo (Ver HTML) |
