| Revista: | Brazilian archives of biology and technology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000392714 |
| ISSN: | 1516-8913 |
| Autors: | Sajidan1 Wulandari, Rita2 Sari, Evy Novita2 Ratriyanto, Adi3 Weldekiros, Hailu4 Greiner, Ralf5 |
| Institucions: | 1Sebelas Maret University, Department of Biology Education, Solo. Indonesia 2Sebelas Maret University, Bioscience Program, Solo. Indonesia 3Sebelas Maret University, Faculty of Agriculture, Solo. Indonesia 4Bahir Dar University, Bahir Dar. Etiopía 5Federal Research Institute of Nutrition and Food, Department of Food Technology and Bioprocess Engineering, Karlsruhe. Alemania |
| Any: | 2015 |
| Període: | Sep-Oct |
| Volum: | 58 |
| Número: | 5 |
| Paginació: | 711-717 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Analítico, descriptivo |
| Resumen en inglés | In this study, 154 isolates capable of producing extracellular phytate - degrading activity were isolated from four soil samples from volcanic areas in Central Java, Indonesia. Six strains with high phytate - degrading activity were selected for strain identification and characterization of the corresponding phytate - degrading enzyme. Blast analysis of 16S rRNA gene sequences revealed high similarities for all the six isolates to reference sequences belonging to the genus Bacillus. Isolates MS5, MC6, D10 and D16 showed 99% sequence identity to B. cereus , while isolate MC8 exhibited 99% sequence identity to B. aryabhatti and D6 99% sequence identity to B. psychrotolerans . The crude extracellular phytase preparations from the isolates showed following optimal conditions for phytate dephosphorylation: pH 4.0 and 50°C (isolate D10), pH 5.0 and 60°C (i solate MC6, and isolate MS5), pH 6.0 and 50°C (isolate D16) and pH 6.0 and 60°C (isolate D6) and pH 6.0 and 40°C (isolate MC8). Zn 2+ and Fe 3+ strongly inhibited phytate dephosphorylation with all phytase preparations studied. In the presence of Ca 2+ , an in crease in phytase activity of 10 - 15% was obtained |
| Disciplines | Biología, Química |
| Paraules clau: | Bacterias, Genética, Bioquímica, Fitasa, Fitatos, Enzimas |
| Keyword: | Biology, Chemistry, Bacteria, Genetics, Biochemistry, Bacillus, Phytase, Phytates, Enzymes |
| Text complet: | Texto completo (Ver PDF) |
